Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRLF1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)
Rabbit CRLF1 Polyclonal Antibody | anti-CRLF1 antibody
CRLF1 Polyclonal Antibody
Gene Names
CRLF1; CLF; NR6; CISS; CISS1; CLF-1; zcytor5
Reactivity
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep
Applications
Immunohistochemistry
Purity
affinity purified by Protein A
Synonyms
CRLF1; Polyclonal Antibody; CRLF1 Polyclonal Antibody; CISS 1; CISS; CISS1; CLF 1; CLF; CLF1; CRLF 1; Cytokine like factor 1; Cytokine receptor like factor 1; cytokine receptor-like factor 1; NR 6; NR6; ZcytoR 5; ZcytoR5; CRLF1_HUMAN; anti-CRLF1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep
Clonality
Polyclonal
Specificity
CRLF1 Polyclonal Antibody detects endogenous levels of CRLF1 protein.
Purity/Purification
affinity purified by Protein A
Form/Format
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-CRLF1 antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:100-500
Immunogen
KLH conjugated synthetic peptide derived from human CRLF1:201-300/422
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRLF1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRLF1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRLF1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRLF1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)
Related Product Information for anti-CRLF1 antibody
CRLF1 is a 422 amino acid secreted protein that interacts with cells expressing ciliary neurotrophic factor receptors. A cytokine receptor subunit belonging to the type I cytokine receptor family and type 3 subfamily, CRLF1 is thought to play a role in fetal nervous system development and immunity. CRLF1 is highly expressed in stomach, placenta, heart, ovary, thyroid, bone marrow, appendix, lymph node, spleen, thymus and fetal lung, and promotes neuronal cell survival. CRLF3 is a 442 amino acid protein that contains one fibronectin type-III domain. CRLF3 is expressed in lesion actinic keratosis (AK) and skin and squamous cell carcinoma (SCC), and is thought to negatively regulate the G0/G1 phase of the cell cycle.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,302 Da
NCBI Official Full Name
cytokine receptor-like factor 1
NCBI Official Synonym Full Names
cytokine receptor-like factor 1
NCBI Official Symbol
CRLF1
NCBI Official Synonym Symbols
CLF; NR6; CISS; CISS1; CLF-1; zcytor5
NCBI Protein Information
cytokine receptor-like factor 1; class I cytokine receptor; cytokine type 1 receptor CRLP-1; cytokine-like factor 1
UniProt Protein Name
Cytokine receptor-like factor 1
Protein Family
UniProt Gene Name
CRLF1
UniProt Synonym Gene Names
CLF-1
UniProt Entry Name
CRLF1_HUMAN
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Product Notes
The CRLF1 crlf1 (Catalog #AAA3005589) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CRLF1 Polyclonal Antibody reacts with Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's CRLF1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:100-500. Researchers should empirically determine the suitability of the CRLF1 crlf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CRLF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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