Rabbit SOX8 Polyclonal Antibody | anti-SOX8 antibody

SOX8 Polyclonal Antibody

Cat. Num. AAA3015231

• Reactivity: Human, Mouse, Rat, Cow, Horse
• Applications: Immunohistochemistry
• Purity: affinity purified by Protein A
• Synonyms: SOX8; Polyclonal Antibody; SOX8 Polyclonal Antibody; SOX 8; Sox8; SOX8_HUMAN; SRY (sex determining region Y) box 8; SRY box 8; SRY box containing gene 8; Transcription factor SOX 8; Transcription factor SOX-8; Transcription factor SOX8; anti-SOX8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Cow, Horse
• Clonality: Polyclonal
• Specificity: SOX8 Polyclonal Antibody detects endogenous levels of SOX8 protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-SOX8 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-500
• Immunogen: KLH conjugated synthetic peptide derived from human SOX8
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX8) Polyclonal Antibody, Unconjugated (bs-11600R) at 1:400 overnight at 4°C)

Related Product Information for anti-SOX8 antibody

Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.

NCBI and Uniprot Product Information

• NCBI GI #: 10720294
• NCBI GeneID: 30812
• UniProt Accession #: P57073; Q9NZW2
• Molecular Weight: 446
• NCBI Official Full Name: Transcription factor SOX-8
• NCBI Official Synonym Full Names: SRY (sex determining region Y)-box 8
• NCBI Official Symbol: SOX8
• NCBI Protein Information: transcription factor SOX-8
• UniProt Protein Name: Transcription factor SOX-8
• Protein Family: Transcription factor
• UniProt Gene Name: SOX8
• UniProt Entry Name: SOX8_HUMAN

NCBI Description

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein may be involved in brain development and function. Haploinsufficiency for this protein may contribute to the mental retardation found in haemoglobin H-related mental retardation (ART-16 syndrome). [provided by RefSeq, Jul 2008]

Uniprot Description

SOX8: May play a role in central nervous system, limb and facial development. May be involved in male sex determination. Binds the consensus motif 5'-[AT][AT]CAA[AT]G-3'.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cytoplasm; nucleus

Molecular Function: DNA binding; protein heterodimerization activity; transcription factor binding

Biological Process: negative regulation of photoreceptor cell differentiation; transcription from RNA polymerase II promoter; fat cell differentiation; cell fate commitment; positive regulation of gliogenesis; in utero embryonic development; astrocyte fate commitment; positive regulation of transcription, DNA-dependent; male gonad development; cell maturation; signal transduction; Sertoli cell development; enteric nervous system development; peripheral nervous system development; osteoblast differentiation; retina development in camera-type eye; positive regulation of osteoblast proliferation; oligodendrocyte differentiation; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of myoblast differentiation; neural crest cell migration; negative regulation of apoptosis