Rabbit CIDEC Polyclonal Antibody | anti-CIDEC antibody

CIDEC Polyclonal Antibody

Cat. Num. AAA3015500

• Gene Names: CIDEC; CIDE3; FPLD5; FSP27; CIDE-3
• Reactivity: Human, Mouse, Rat, Pig
• Applications: Immunohistochemistry
• Purity: affinity purified by Protein A
• Synonyms: CIDEC; Polyclonal Antibody; CIDEC Polyclonal Antibody; Cell Death Activator; Cell death activator CIDE-3; Cell Death Inducing DFFA Like Effector C; Cell death inducing DFFA like effector protein C; Cell death-inducing DFFA-like effector protein C; CIDE 3; CIDE3; CIDE C; CIDEC_HUMAN; Fat specific protein 27; Fat-specific protein FSP27 homolog; FLJ20871; FSP27; anti-CIDEC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Pig
• Clonality: Polyclonal
• Specificity: CIDEC Polyclonal Antibody detects endogenous levels of CIDEC protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-CIDEC antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-500
• Immunogen: KLH conjugated synthetic peptide derived from human CIDEC:101-200/238
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (rat breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CIDEC) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Related Product Information for anti-CIDEC antibody

This gene encodes a member of the cell death-inducing DNA fragmentation factor-like effector family. Members of this family play important roles in apoptosis. The encoded protein promotes lipid droplet formation in adipocytes and may mediate adipocyte apoptosis. This gene is regulated by insulin and its expression is positively correlated with insulin sensitivity. Mutations in this gene may contribute to insulin resistant diabetes. A pseudogene of this gene is located on the short arm of chromosome 3. Alternatively spliced transcript variants that encode different isoforms have been observed for this gene

NCBI and Uniprot Product Information

• NCBI GI #: 313851088
• NCBI GeneID: 63924
• NCBI Accession #: NP_001186480.1
• NCBI GenBank Nucleotide #: NM_001199551.1
• UniProt Accession #: Q96AQ7; Q67DW9; Q9GZY9; C9JMN7
• Molecular Weight: 27,923 Da
• NCBI Official Full Name: cell death activator CIDE-3 isoform 2
• NCBI Official Synonym Full Names: cell death inducing DFFA like effector c
• NCBI Official Symbol: CIDEC
• NCBI Official Synonym Symbols: CIDE3; FPLD5; FSP27; CIDE-3
• NCBI Protein Information: cell death activator CIDE-3
• UniProt Protein Name: Cell death activator CIDE-3
• Protein Family: Cell death activator
• UniProt Gene Name: CIDEC
• UniProt Synonym Gene Names: FSP27
• UniProt Entry Name: CIDEC_HUMAN

Uniprot Description

CIDEC: May act as a CEBPB coactivator in white adipose tissue to control the expression of a subset of CEBPB downstream target genes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH. Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair. When overexpressed in preadipocytes, induces apoptosis or increases cell susceptibility to apoptosis induced by serum deprivation or TGFB treatment. As mature adipocytes, that express high CIDEC levels, are quite resistant to apoptotic stimuli, the physiological significance of its role in apoptosis is unclear. In omental adipose tissue of obese patients matched for BMI, expression levels tend to correlate with insulin sensitivity. Expression is increased 2-3 fold in the group of patients with high insulin sensitivity, compared to the insulin- resistant group. This observation is consistent with the idea that triglyceride storage in adipocytes plays an important role in sequestering triglycerides and fatty acids away from the circulation and peripheral tissues, thus enhancing insulin sensitivity in liver and muscle. This effect is not significant in subcutaneous adipose tissue (PubMed:18509062). In subcutaneous adipose tissue of diabetic patients, tends to negatively correlate with body mass index and total fat mass, independently of insulin sensitivity (PubMed:18334488). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: cytosol; lipid particle

Biological Process: apoptosis; regulation of apoptosis

Disease: Lipodystrophy, Familial Partial, Type 5