Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FMN1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)
Rabbit FMN1 Polyclonal Antibody | anti-FMN1 antibody
FMN1 polyclonal antibody
Gene Names
FMN1; LD; FMN
Reactivity
Human, Mouse, Rat, Dog, Cow, Sheep
Applications
Immunohistochemistry
Purity
affinity purified by Protein A
Synonyms
FMN1; Polyclonal Antibody; FMN1 polyclonal antibody; FMN; Formin 1; Formin1; Formin-1; LD; Limb deformity protein homolog; FMN1_HUMAN.?; anti-FMN1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Cow, Sheep
Clonality
Polyclonal
Specificity
FMN1 Polyclonal Antibody detects endogenous levels of FMN1 protein.
Purity/Purification
affinity purified by Protein A
Form/Format
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-FMN1 antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:100-500
Immunogen
KLH conjugated synthetic peptide derived from human FMN1/Formin 1:651-750/1419
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FMN1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FMN1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FMN1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FMN1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FMN1) Polyclonal Antibody, Unconjugated (bs-13185R) at 1:200 overnight at 4°C)
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FMN1) Polyclonal Antibody, Unconjugated (bs-13185R) at 1:200 overnight at 4°C)
Related Product Information for anti-FMN1 antibody
The temporal genetic hierarchy influencing normal limb development can deregulate and mediate mammalian developmental syndromes. In mice, the limb deformity (ld) locus influences normal limb development and gives rise to alternative mRNAs that can translate into a family of proteins known as formins. Formins play a crucial role in cytoskeletal reorganization by influencing Actin filament assembly. Formins co-localize with the actin cytoskeleton and can translocate into the cell cytosol and into the nucleus in an HGF-dependent manner. Vertebrate nuclear formins can control polarizing activity in limb buds through establishment of a Sonic hedgehog/FGF-4 feedback loop. Deficiency mutations at the mammalian ld locus lead to profound developmental defects in limb and kidney formation. The human Formin 1 and 2 genes map to chromosome 15q13.3 and 1q43, respectively.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
157,578 Da
NCBI Official Full Name
formin-1 isoform b
NCBI Official Synonym Full Names
formin 1
NCBI Official Symbol
FMN1
NCBI Official Synonym Symbols
LD; FMN
NCBI Protein Information
formin-1; formin (limb deformity); limb deformity protein homolog
UniProt Protein Name
Formin-1
Protein Family
UniProt Gene Name
FMN1
UniProt Synonym Gene Names
FMN; LD
UniProt Entry Name
FMN1_HUMAN
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Product Notes
The FMN1 fmn1 (Catalog #AAA3015285) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FMN1 polyclonal antibody reacts with Human, Mouse, Rat, Dog, Cow, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's FMN1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:100-500. Researchers should empirically determine the suitability of the FMN1 fmn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FMN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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