Rabbit anti-Rat GATA Binding Protein 4 (GATA4) Polyclonal Antibody | anti-GATA4 antibody

Polyclonal Antibody to GATA Binding Protein 4 (GATA4)

Cat. Num. AAA2004291

• Reactivity: Rat
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: GATA Binding Protein 4 (GATA4); Polyclonal Antibody; Polyclonal Antibody to GATA Binding Protein 4 (GATA4); anti-GATA4 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against GATA4. It has been selected for its ability to recognize GATA4 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-RRD GTGHYLCNAC GLYHKMNGIN RPLIKPQRRL SASRRVGLSC ANCQTTTTTL WRRNAEGEPV CNACGLYMKL HGVPRPLAMR KEGIQTRKRK PKNLNKSKTP AGPPGESLPP SSGASSNSSN ATSSSSSSEE MRPIKTEPGL SSHYGHSSSM SQTFSTVSGH GSSIHPVLSA LKLSPQGYPS PVTQTSQASS KQDSWNSLVL ADSHGDIITA
• Sequence Length: 442

• Applicable Applications for anti-GATA4 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant GATA4 (Arg228~Ala440) expressed in E.coli.
• Cross Reactivity: Rat
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2041582
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant GATA4, Rat.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Spleen Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Lung Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Kidney Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Stomach Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 25282465
• NCBI GeneID: 54254
• NCBI Accession #: NP_653331.1
• NCBI GenBank Nucleotide #: NM_144730.1
• UniProt Accession #: P46152
• Molecular Weight: 44,597 Da
• NCBI Official Full Name: transcription factor GATA-4
• NCBI Official Synonym Full Names: GATA binding protein 4
• NCBI Official Symbol: Gata4
• NCBI Protein Information: transcription factor GATA-4
• UniProt Protein Name: Transcription factor GATA-4
• Protein Family: GATA transcription factor
• UniProt Gene Name: Gata4

NCBI Description

plays a role in regulation of cardiac-myocyte specific gene expression; interacts with dHAND (HAND2) to activate transcription of several cardiac-specific genes [RGD, Feb 2006]

Uniprot Description

GATA4: a conserved and ubiquitous member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. May regulate a set of cardiac-specific genes and play a crucial role in cardiogenesis. Defects are a cause of atrial septal defect 2 (ASD2). ASD2 is an autosomal dominant condition with atrial septal defect and other congenital heart disease but no conduction defects or noncardiac abnormalities.

Protein type: Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 15p12

Cellular Component: nuclear body; nuclear chromatin; nucleus; RNA polymerase II transcription factor complex; transcription factor complex

Molecular Function: activating transcription factor binding; chromatin binding; co-SMAD binding; DNA binding; DNA binding transcription factor activity; NFAT protein binding; protein kinase binding; sequence-specific DNA binding; transcription coactivator activity; transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding; transcription factor binding; transcription regulatory region DNA binding; zinc ion binding

Biological Process: atrial septum primum morphogenesis; atrial septum secundum morphogenesis; atrioventricular valve formation; atrioventricular valve morphogenesis; BMP signaling pathway; cardiac muscle cell differentiation; cardiac muscle hypertrophy in response to stress; cardiac muscle tissue development; cardiac right ventricle morphogenesis; cardiac septum development; cell-cell signaling; cellular response to follicle-stimulating hormone stimulus; cellular response to glucose stimulus; cellular response to gonadotropin stimulus; embryonic digestive tract morphogenesis; embryonic foregut morphogenesis; embryonic heart tube anterior/posterior pattern formation; embryonic heart tube development; embryonic morphogenesis; endocardial cushion development; endoderm formation; epithelial cell fate commitment; gastrulation with mouth forming second; heart development; heart looping; heart morphogenesis; in utero embryonic development; male gonad development; negative regulation of apoptosis; negative regulation of apoptotic signaling pathway; negative regulation of autophagy; negative regulation of cardiac muscle cell apoptotic process; negative regulation of connective tissue replacement; negative regulation of gene expression; positive regulation of angiogenesis; positive regulation of BMP signaling pathway; positive regulation of cardiac muscle cell proliferation; positive regulation of cardioblast differentiation; positive regulation of cell cycle; positive regulation of ERK1 and ERK2 cascade; positive regulation of gene expression; positive regulation of protein phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of vascular endothelial growth factor production; regulation of cardiac muscle cell contraction; regulation of cardiac muscle cell proliferation; regulation of gene expression; regulation of protein kinase B signaling; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; response to drug; response to estrogen; response to mechanical stimulus; response to retinoic acid; response to vitamin A; seminiferous tubule development; Sertoli cell differentiation; spermatogenesis; transcription from RNA polymerase II promoter; tube morphogenesis; ventricular cardiac muscle tissue development; ventricular septum development; Wnt receptor signaling pathway through beta-catenin