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Western Blot (WB) ((Figure. Western Blot; Sample: Recombinant protein.))

Rabbit anti-Horse Interferon Beta (IFNb) Polyclonal Antibody | anti-IFNb antibody

Polyclonal Antibody to Interferon Beta (IFNb)

Gene Names
IFNB1; IFB; IFF; IFNB
Reactivity
Horse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Interferon Beta (IFNb); Polyclonal Antibody; Polyclonal Antibody to Interferon Beta (IFNb); anti-IFNb antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Horse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against IFNb. It has been selected for its ability to recognize IFNb in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-VNYDLLRSQ LRSSNSACLM LLRQLNGAPQ RCPEDTMNFQ VPEEIEQAQQ FQKEDAALVI YEMLQHTWRI FRRNFASTGW NETIVKNLLV EVHLQMDRLE TNLEEIMEEE SSTWGNTTIL RLKKYYGRIS QYLKAKKYSH CAWTVVQAEM LRNLAFLNGL TDYLQN
Sequence Length
187
Applicable Applications for anti-IFNb antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant IFNb (Val22~Asn186) expressed in E.coli.
Cross Reactivity
Horse
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

((Figure. Western Blot; Sample: Recombinant protein.))

Western Blot (WB) ((Figure. Western Blot; Sample: Recombinant protein.))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,294 Da
NCBI Official Full Name
interferon beta
NCBI Official Synonym Full Names
interferon, beta 1, fibroblast
NCBI Official Symbol
IFNB1
NCBI Official Synonym Symbols
IFB; IFF; IFNB
NCBI Protein Information
interferon beta; IFN-beta; fibroblast interferon
UniProt Protein Name
Interferon beta
Protein Family
UniProt Gene Name
IFNB1
UniProt Synonym Gene Names
IFB; IFNB; IFN-beta
UniProt Entry Name
IFNB_HUMAN

Uniprot Description

IFNB1: Has antiviral, antibacterial and anticancer activities. Belongs to the alpha/beta interferon family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: extracellular space; extracellular region

Molecular Function: interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; response to virus; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; natural killer cell activation; negative regulation of T cell differentiation; humoral immune response; T cell activation during immune response; cell surface receptor linked signal transduction; response to exogenous dsRNA; negative regulation of viral genome replication; B cell differentiation; positive regulation of innate immune response; defense response to bacterium; natural killer cell activation during immune response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; defense response to virus; B cell activation during immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein

Research Articles on IFNb

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Product Notes

The IFNb ifnb1 (Catalog #AAA2002551) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Interferon Beta (IFNb) reacts with Horse and may cross-react with other species as described in the data sheet. AAA Biotech's Interferon Beta (IFNb) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the IFNb ifnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-VNY DLLRSQ LRSSNSACLM LLRQLNGAPQ RCPEDTMNFQ VPEEIEQAQQ FQKEDAALVI YEMLQHTWRI FRRNFASTGW NETIVKNLLV EVHLQMDRLE TNLEEIMEEE SSTWGNTTIL RLKKYYGRIS QYLKAKKYSH CAWTVVQAEM LRNLAFLNGL TDYLQN. It is sometimes possible for the material contained within the vial of "Interferon Beta (IFNb), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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