Rabbit anti-Human, rat Fractin Polyclonal Antibody | anti-fragment of Actin antibody

Anti-Fractin

Cat. Num. AAA502149

• Reactivity: Human, rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Neat Serum (neat serum.)
• Synonyms: Fractin; Polyclonal Antibody; Anti-Fractin; anti-fragment of Actin antibody

• Host: Rabbit
• Reactivity: Human, rat
• Clonality: Polyclonal
• Specificity: Specific for the ~ 32 kDa fractin protein in Western blots with no reactivity to intact actin. There is often a ladder of smaller bands in cells or culture or in vivo preparations due to further degradation by other proteases.
• Purity/Purification: Neat Serum (neat serum.)
• Form/Format: Liquid. Contains no preservative.

• Applicable Applications for anti-fragment of Actin antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1,000; IHC: 1:100
• Antigen: Synthetic peptide taken from the C terminus of the 32-kDa actin fragment.
• Immunogen Information: Synthetic peptide corresponding to amino acid residues from the C terminal region of the 32-kDa actin fragment
• Immunogen Species: Human
• Species Reactivity Note: The antibody has been directly tested for reactivity in human and rat.
• Biological Significance: Fractin (fragment of actin) is a caspase-specific cleavage product of actin and serves as a novel marker of apoptosis-related events. The antibody has been shown to detect the processes and cell bodies of degenerating neurons and plaque-associated microglia in Alzheimer's disease (Yang et al., 1998). It has recently been reported that Fractin may have a functional role in apoptotic signaling in oligodendrocytes (Schulz, R., et al., Glia, 2009, in press).
• Preparation and Storage: Store at -20 degree C in undiluted aliquots; stable for at least 1 year from date of receipt. Avoid freeze/thaw cycles.

Testing Data

Related Product Information for anti-fragment of Actin antibody

Rabbit polyclonal antibody

References

• Yang, F, et al., Antibody to Caspase-Cleaved Actin Detects Apoptosis in Differentiated Neuroblastoma and Plaque-Associated Neurons and Microglia in Alzheimer's disease. American Journal of Pathology, 1998, Vol. 152, No. 2, p. 379-389.
• Rossiter, JP, et al., Caspase-cleaved actin (fractin) immunolabeling of Hirano bodies. Neuropathol Appl Neurobiol. 2000 Aug; 26(4):342-6.
• Asamec, E, et al., Multiple-label immunocytochemistry for the evaluation of nature of cell death in experimental models of neurodegeneration. Brain Res Protoc. 2001 Jul;7(3):193-202.
• Chen, TA, et al., Inhibition of caspase-3-like activity reduces glutamate induced cell death in adult rat retina. Brain Res. 2001 Jun 15;904(1):177-88.
• Rossiter, JP, et al., Caspase-3 activation and caspase-like proteolytic activity in human perinatal hypoxic-ischemic brain injury. Acta Neuropathol. 2002 Jan;103(1):66-73.