Rabbit anti-Rat PEBP (phosphatidylethanolamie binding protein) Polyclonal Antibody | anti-PEBP1 antibody

Anti-PEBP (Phosphatidylethanolamine Binding Protein)

Cat. Num. AAA502101

• Gene Names: Pebp1; Pbp; HCNP; Rkip
• Reactivity: Rat
• Applications: Western Blot
• Purity: Neat Serum
• Synonyms: PEBP (phosphatidylethanolamie binding protein); Polyclonal Antibody; Anti-PEBP (Phosphatidylethanolamine Binding Protein); anti-PEBP1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~ 22k PEBP protein in Western blots
• Purity/Purification: Neat Serum
• Form/Format: 100 ul neat serum
• Sequence Length: 187

• Applicable Applications for anti-PEBP1 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Native PEBP protein purified from rat
• Immunogen Information: Purified native rat PEBP
• Immunogen Species: Rat
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blot with rat tissues.
• Biological Significance: Phosphatidylethanolamine binding protein (PEBP), also known as Raf kinase inhibitory protein (RKIP) is a highly conserved multifunctional protein that modulates the Raf/MAPK signaling cascade and has been implicated as a suppressor of metastatic cancer (Trakul N et al., 2005). PEBP has been shown to not only inhibit cell proliferation but also to induce differentiation of human keratinocytes (Yamazaki et al., 2004). Dysregulation of calpain activity results in cellular degeneration in conditions such as Alzheimer's disease and recently PEBP has been identified as a potential in vivo calpain substrate (Chen at al., 2006).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-PEBP1 antibody

Rabbit polyclonal antibody

References

• Trakul N, Menard RE, Schade GR, Qian Z, Rosner MR (2005) Raf kinase inhibitory protein regulates Raf-1 but not BRaf kinase activation. J. Biol. Chem. 280(26): 24931-40.
• Yamazaki T, Nakano H, Hayakari M, Tanaka M, Mayama J, Tsuchida S (2004) Differentiation induction of human keratinocytes by phosphatidylethanolamine binding protein. J. Biol. Chem. 279(31):32191-95.
• Chen Q, Wang S, Thompson SN, Hall ED, Guttmann RP (2006) Identification and characterization of PEBP as a calpain substrate. J Neurochem. 99(4): 1133-41.

NCBI and Uniprot Product Information

• NCBI GI #: 8393910
• NCBI GeneID: 29542
• NCBI Accession #: NP_058932.1
• NCBI GenBank Nucleotide #: NM_017236.1
• UniProt Accession #: P31044; P31045
• Molecular Weight: 22
• NCBI Official Full Name: phosphatidylethanolamine-binding protein 1
• NCBI Official Synonym Full Names: phosphatidylethanolamine binding protein 1
• NCBI Official Symbol: Pebp1
• NCBI Official Synonym Symbols: Pbp; HCNP; Rkip
• NCBI Protein Information: phosphatidylethanolamine-binding protein 1; P23K; HCNPpp; PEBP-1; Raf-1 kinase inhibitor protein; 23 kDa morphine-binding protein; hippocampal cholinergic neurostimulating peptide
• UniProt Protein Name: Phosphatidylethanolamine-binding protein 1
• Protein Family: Phosphatidylethanolamine-binding protein
• UniProt Gene Name: Pebp1
• UniProt Synonym Gene Names: Pbp; Pebp; PEBP-1; HCNP
• UniProt Entry Name: PEBP1_RAT

NCBI Description

lipid binding protein; enhances acetylcholine synthesis in medial septal regions; human homolog (RKIP) has been shown to inhibit MEK- and ERK- signalling pathways [RGD, Feb 2006]

Uniprot Description

Function: Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase

By similarity. Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation

By similarity.HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.

Subunit structure: Has a tendency to form dimers by disulfide cross-linking. Interacts with RAF1 and this interaction is enhanced if RAF1 is phosphorylated on residues 'Ser-338', 'Ser-339', 'Tyr-340' and 'Tyr-341'. Interacts with ALOX15; in response to IL13/interleukin-13, prevents the interaction of PEBP1 with RAF1 to activate the ERK signaling cascade

By similarity.

Subcellular location: Cytoplasm. Membrane; Peripheral membrane protein.

Tissue specificity: Major component of epididymal secretions and sperm plasma membranes. It is present in cytosols from a variety of other tissues. Highly expressed in brain.

Miscellaneous: Seems to be associated with memory and learning disorder.

Sequence similarities: Belongs to the phosphatidylethanolamine-binding protein family.