Rabbit anti-Rat C5a Polyclonal Antibody | anti-C5a antibody

C5a, NT (Anaphylatoxin C5a Analog, Anaphylatoxin C5a, C5, C5a, Complement C5, Complement Component C5, Complement Component C5a, CPAMD 4, CPAMD4) (APC)

Cat. Num. AAA6460695

• Gene Names: C5; Hc; C5a; RGD1561905
• Reactivity: Rat
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: C5a; Polyclonal Antibody; NT (Anaphylatoxin C5a Analog; Anaphylatoxin C5a; C5; Complement C5; Complement Component C5; Complement Component C5a; CPAMD 4; CPAMD4) (APC); anti-C5a antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat C5a.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-C5a antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to aa59-77, TIADKIRKESHHKGMLLGR at the C-terminal, of rat C5a. The sequence is different in mouse by five amino acids.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months after receipt at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(All lanes: 303165 at 0.5ug/ml Lane 1: Rat Liver Tissue Lysate at 40ug Lane 2: Mouse Liver Tissue Lysate at 40ug Lane 3: NIH Whole Cell Lysate at 40ug Lane 4: HEPA Whole Cell Lysate at 40ug Lane 5: PC12 Whole Cell Lysate at 40ug Predicted band size: 115kD Observed band size: 115kD)

Testing Data

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Related Product Information for anti-C5a antibody

C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.

Product Categories/Family for anti-C5a antibody

Antibodies; Complement

References

1. Delgado-Cervino, E., Fontan, G., Lopez-Trascara, M. C5 complement deficiency in a Spanish family: molecular characterization of the double mutation responsible for the defect. Molec. Immun. 42: 105-111, 2005. 2. Pfarr, N., Prawitt, D., Kirschfink, M., Schroff, C., Knuf, M., Habermehl, P., Mannhardt, W., Zepp, F., Fairbrother, W. G., Loos, M., Burge, C. B., Pohlenz, J. Linking C5 deficiency to an exonic splicer enhancer mutation. J. Immun. 174: 4172-4177, 2005. Note: Erratum: J. Immun. 182: 5152 only, 2009.

NCBI and Uniprot Product Information

• NCBI GI #: 46397770
• NCBI GeneID: 362119
• UniProt Accession #: P08650; Q63078
• Molecular Weight: 8,981 Da
• NCBI Official Full Name: Complement C5
• NCBI Official Synonym Full Names: complement component 5
• NCBI Official Symbol: C5
• NCBI Official Synonym Symbols: Hc; C5a; RGD1561905
• NCBI Protein Information: complement C5; anaphylatoxin
• UniProt Protein Name: Complement C5
• Protein Family: C5a peptidase
• UniProt Gene Name: C5
• UniProt Entry Name: CO5_RAT

NCBI Description

likely component of the classical pathway of the complement cascade; may be involved with tissue injury associated with ischemia or sepsis; may be a component of the membrane attack complex [RGD, Feb 2006]

Uniprot Description

C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space

Molecular Function: C5a anaphylatoxin chemotactic receptor binding

Biological Process: cellular calcium ion homeostasis; positive regulation of angiogenesis; positive regulation of chemotaxis; in utero embryonic development; complement activation, alternative pathway; glucose homeostasis; chemotaxis; inflammatory response; negative regulation of dopamine secretion; complement activation, classical pathway; leukocyte migration during inflammatory response