Testing Data
(3T3-L1 cells were stained with MBS613475 (filled histogram), or control antibody (open histogram) followed by APC-conjugated streptavidin.)
Sheep anti-Mouse FPRP Polyclonal Antibody
FPRP (F2alpha Prostaglandin Receptor Regulatory Protein, CD315, EWI-F, CD9 Partner-1, CD9P-1) (Biotin)
Reactivity
Mouse
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
FPRP; Polyclonal Antibody; FPRP (F2alpha Prostaglandin Receptor Regulatory Protein; CD315; EWI-F; CD9 Partner-1; CD9P-1) (Biotin); Anti -FPRP (F2alpha Prostaglandin Receptor Regulatory Protein; anti-FPRP antibody
Host
Sheep
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse FPRP extracellular domain.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 1ml sterile Tris buffered saline, 0.1% BSA.
Applicable Applications for anti-FPRP antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot and Flow Cytometry.
Dilution: Western Blot: 0.1-0.2ug/ml The detection limit is ~5ng/lane under non-reducing and reducing conditions.
Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 3T3-L1 cells in a total reaction volume 200ul.
Dilution: Western Blot: 0.1-0.2ug/ml The detection limit is ~5ng/lane under non-reducing and reducing conditions.
Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 3T3-L1 cells in a total reaction volume 200ul.
Immunogen
NS0-derived recombinant mouse FPRP extracellular domain (aa22-832).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile Tris buffered saline, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(3T3-L1 cells were stained with MBS613475 (filled histogram), or control antibody (open histogram) followed by APC-conjugated streptavidin.)
Testing Data
(3T3-L1 cells were stained with MBS613475 (filled histogram), or control antibody (open histogram) followed by APC-conjugated streptavidin.)
Related Product Information for anti-FPRP antibody
FPRP (F2alpha Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130kD type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F2alpha receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton. Mature mouse FPRP is 858 amino acids (aa) in length. Its extracellular domain (ECD) contains six C2-type Ig-like domains (aa22-813) plus a Pro-Thr-Glu-Leu ER retention signal. The ECD of mouse FPRP (aa22-832) shares 89% and 98% aa identity with the ECD of human and rat FPRP, respectively.
Product Categories/Family for anti-FPRP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
F2 alpha prostoglandin regulatory protein
Uniprot Description
PTGFRN: Inhibits the binding of prostaglandin F2-alpha (PGF2- alpha) to its specific FP receptor, by decreasing the receptor number rather than the affinity constant. Functional coupling with the prostaglandin F2-alpha receptor seems to occur.
Protein type: Membrane protein, integral; Cytoskeletal
Cellular Component: Golgi apparatus; cell surface; membrane; endoplasmic reticulum; integral to membrane
Similar Products
Product Notes
The FPRP (Catalog #AAA613475) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The FPRP (F2alpha Prostaglandin Receptor Regulatory Protein, CD315, EWI-F, CD9 Partner-1, CD9P-1) (Biotin) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FPRP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot and Flow Cytometry. Dilution: Western Blot: 0.1-0.2ug/ml The detection limit is ~5ng/lane under non-reducing and reducing conditions. Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 3T3-L1 cells in a total reaction volume 200ul. Researchers should empirically determine the suitability of the FPRP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FPRP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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