Goat anti-Human Plexin D1 Polyclonal Antibody | anti-PLXND1 antibody

Plexin D1

Cat. Num. AAA611287

• Gene Names: PLXND1; PLEXD1; KIAA0620; MGC75353
• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Plexin D1; Polyclonal Antibody; Anti -Plexin D1; anti-PLXND1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Plexin D1. Species sequence homology: mouse 89% and canine 91%.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.

• Applicable Applications for anti-PLXND1 antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry, Western Blot and ELISA.; Dilution: Flow Cytometry: 25ug/ml. Add 10ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul.; Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane under non-reducing and reducing conditions.; Direct ELISA: 0.5-1ug/ml. The detection limit is ~0.2ng/well.
• Immunogen: NS0-derived recombinant human Plexin D1 extracellular domain (aa47-1271) Accession # Q9Y4D7
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, PBS. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Detection of Human Plexin D1 by Western Blot.)

Related Product Information for anti-PLXND1 antibody

Plexin D1 is a type I transmembrane protein that is the prototype of the Plexin D subfamily in the Plexin family. It is expressed on multiple tissues including fetal neuronal and endothelial cells. Plexin D1 expression is also induced on tumor vasculature and tumor cells. The 1879 amino acid (aa) mature human Plexin D1 contains a large (1225 aa) extracellular domain with a 500 aa sema domain and three short MRS (Met-related sequences) cysteine-rich motifs. By alternative splicing, multiple Plexin-D1 isoforms may exist. Plexin D1 binds Sema4A to suppress angiogenesis. It also binds Sema3E directly to mediate repulsive vascular vessal guidance. In mouse, this interaction has been shown to be independent of Neuropilin and Sema3A. Over the extracellular region used as immunogen, human Plexin D1 shares 89% and 91% aa identity with the mouse and canine protein, respectively.

Product Categories/Family for anti-PLXND1 antibody

Antibodies; Abs to Plexin; Semaphorin

NCBI and Uniprot Product Information

• NCBI GI #: 157694524
• NCBI GeneID: 23129
• NCBI Accession #: NP_055918.2
• NCBI GenBank Nucleotide #: NM_015103.2
• UniProt Accession #: Q9Y4D7; Q6PJS9; Q8IZJ2; Q9BTQ2; A7E2C6; C9JPZ6; Q6P657; Q86YC4
• Molecular Weight: 212,007 Da
• NCBI Official Full Name: plexin-D1
• NCBI Official Synonym Full Names: plexin D1
• NCBI Official Symbol: PLXND1
• NCBI Official Synonym Symbols: PLEXD1; KIAA0620; MGC75353
• NCBI Protein Information: plexin-D1; OTTHUMP00000215785
• UniProt Protein Name: Plexin-D1
• Protein Family: Plexin
• UniProt Gene Name: PLXND1
• UniProt Synonym Gene Names: KIAA0620
• UniProt Entry Name: PLXD1_HUMAN

Uniprot Description

PLXND1: Cell surface receptor for SEMA4A and for class 3 semaphorins, such as SEMA3A, SEMA3C and SEMA3E. Plays an important role in cell-cell signaling, and in regulating the migration of a wide spectrum of cell types. Regulates the migration of thymocytes in the medulla. Regulates endothelial cell migration. Plays an important role in ensuring the specificity of synapse formation. Required for normal development of the heart and vasculature. Mediates anti-angiogenic signaling in response to SEMA3E. Belongs to the plexin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: integral to plasma membrane; lamellipodium; plasma membrane; intracellular

Molecular Function: protein domain specific binding; protein binding; semaphorin receptor activity

Biological Process: patterning of blood vessels; axon guidance; synaptogenesis; positive regulation of protein binding; endothelial cell migration; angiogenesis; regulation of angiogenesis; regulation of cell migration