Testing Data
(Mouse splenocytes were stained with MBS610825 (filled histogram) or control antibody (open histogram) followed by NL637-conjugated anti-sheep antibody.)
Sheep anti-Mouse, Rat Delta-like 1 Polyclonal Antibody | anti-DLL3 antibody
Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172)
Gene Names
DLL3; SCDO1
Reactivity
Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Immunoaffinity chromatography.
Synonyms
Delta-like 1; Polyclonal Antibody; Delta-like 1 (DLL1; Delta1; DL1; H-Delta-1; UNQ146/PRO172); Anti -Delta-like 1 (DLL1; anti-DLL3 antibody
Host
Sheep
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes the extracellular domain of rat Delta-like 1. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by Immunoaffinity chromatography.
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Applicable Applications for anti-DLL3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, ELISA and Flow Cytometry.
Dilution: Western Blot: 0.1-0.2ug/ml; The detection limit is 5ng/lane under non-reducing and 25ng/lane under reducing conditions.
Immunohistochemistry: 10ug/ml detects DLL1 in cryosections of E13.5 mouse embryos. Tissue was fixed overnight in 4% PFA+14% picric acid in PBS at 4 degree C. Sections were blocked with 10% donkey serum and 0.1% Triton in 1% BSA in PBS for 45 min at RT. After blocking, sections were incubated with D2727-20 for 3 hours at RT followed by fluorochrome-coupled anti-sheep lgG at RT for 1 hour. Between each step, sections were washed with 0.1% BSA in PBS.
Direct ELISA: 0.5-1.0ug/ml; The detection limit is 0.3ng/well.
Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 cells in total reaction volume < 200ul. Validated using mouse splenocytes.
Optimal dilutions to be determined by researcher.
Dilution: Western Blot: 0.1-0.2ug/ml; The detection limit is 5ng/lane under non-reducing and 25ng/lane under reducing conditions.
Immunohistochemistry: 10ug/ml detects DLL1 in cryosections of E13.5 mouse embryos. Tissue was fixed overnight in 4% PFA+14% picric acid in PBS at 4 degree C. Sections were blocked with 10% donkey serum and 0.1% Triton in 1% BSA in PBS for 45 min at RT. After blocking, sections were incubated with D2727-20 for 3 hours at RT followed by fluorochrome-coupled anti-sheep lgG at RT for 1 hour. Between each step, sections were washed with 0.1% BSA in PBS.
Direct ELISA: 0.5-1.0ug/ml; The detection limit is 0.3ng/well.
Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 cells in total reaction volume < 200ul. Validated using mouse splenocytes.
Optimal dilutions to be determined by researcher.
Immunogen
Recombinant protein corresponding to aa18-537 of rat Delta-like 1 expressed in NSO cells (Accession #: P97677). Species Sequence Homology: mouse (97%), human (91%).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Mouse splenocytes were stained with MBS610825 (filled histogram) or control antibody (open histogram) followed by NL637-conjugated anti-sheep antibody.)
Testing Data
(Mouse splenocytes were stained with MBS610825 (filled histogram) or control antibody (open histogram) followed by NL637-conjugated anti-sheep antibody.)
Related Product Information for anti-DLL3 antibody
Delta-1 (also known as Delta-like 1, DLL1) is an 80-100kD vertebrate homolog of the Drosophila delta protein. Rat Delta-1 is a type I transmembrane protein that is 697aa in length. It contains a 520aa extracellular domain (ECD) and a 154aa cytoplasmic region. The ECD has one DSL segment (aa158-220) and eight consecutive EGF-like domains (aa225-515). Proteolytic processing of Delta-1 by ADAMs generates soluble intracellular and extracellular domains. Delta-1 binds to Notch and limits monocyte development while promoting T cell and DC development.
Product Categories/Family for anti-DLL3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,618 Da
NCBI Official Full Name
delta-like protein 3 isoform 1
NCBI Official Synonym Full Names
delta-like 3 (Drosophila)
NCBI Official Symbol
DLL3
NCBI Official Synonym Symbols
SCDO1
NCBI Protein Information
delta-like protein 3; delta3; drosophila Delta homolog 3
UniProt Protein Name
Delta-like protein 3
Protein Family
UniProt Gene Name
DLL3
UniProt Synonym Gene Names
Delta3
UniProt Entry Name
DLL3_HUMAN
NCBI Description
This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
DLL3: Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. Can bind and activate Notch-1 or another Notch receptor.
Protein type: Cell development/differentiation; Membrane protein, integral
Chromosomal Location of Human Ortholog: 19q13
Cellular Component: integral to membrane
Molecular Function: Notch binding
Biological Process: compartment specification; paraxial mesoderm development; somitogenesis; Notch signaling pathway; skeletal development; negative regulation of neurogenesis
Disease: Spondylocostal Dysostosis 1, Autosomal Recessive
Research Articles on DLL3
Similar Products
Product Notes
The DLL3 dll3 (Catalog #AAA610825) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Delta-like 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, ELISA and Flow Cytometry. Dilution: Western Blot: 0.1-0.2ug/ml; The detection limit is 5ng/lane under non-reducing and 25ng/lane under reducing conditions. Immunohistochemistry: 10ug/ml detects DLL1 in cryosections of E13.5 mouse embryos. Tissue was fixed overnight in 4% PFA+14% picric acid in PBS at 4 degree C. Sections were blocked with 10% donkey serum and 0.1% Triton in 1% BSA in PBS for 45 min at RT. After blocking, sections were incubated with D2727-20 for 3 hours at RT followed by fluorochrome-coupled anti-sheep lgG at RT for 1 hour. Between each step, sections were washed with 0.1% BSA in PBS. Direct ELISA: 0.5-1.0ug/ml; The detection limit is 0.3ng/well. Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 cells in total reaction volume < 200ul. Validated using mouse splenocytes. Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the DLL3 dll3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Delta-like 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
