Western Blot (WB)
(Western blot of connexin35/36 fusion proteins showing specific immunolabeling of the ~35 kDa connexin-35/36 phosphorylated at Ser276. The first lane (1) is PKA phosphorylated protein purified from the C-Terminal domain. The second lane (2) shows phosphospecificity indicated by no labeling in protein without PKA stimulation. The third lane (3) is protein purified from the intracellular loop, demonstrating that there is no cross-reactivity with the other major PKA phosphorylation site at Ser110.)
Rabbit Connexin35/36 Polyclonal Antibody | anti-Cx35 antibody
Phospho-Connexin35/36 (Ser276)
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified from Pooled Serum
Synonyms
Connexin35/36; Polyclonal Antibody; Phospho-Connexin35/36 (Ser276); Connexin 36/GJA9 antibody; Connexin 36 antibody; Connexin-36 antibody; Cx36 antibody; CXD2_HUMAN antibody; Gap junction alpha-9 protein antibody; Gap junction delta-2 protein antibody; Gap junction protein alpha 9 antibody; gap junction protein; alpha 9; 36kDa antibody; GJA9 antibody; GJD2 antibody; C138315 antibody; MGC138319 antibody; Connexin 35 antibody; Connexin-35 antibody; Cx35 antibody; Cx35.4 antibody; anti-Cx35 antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified from Pooled Serum
Form/Format
100ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Applicable Applications for anti-Cx35 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000
IHC: 1:500
IHC: 1:500
Species Positively Tested
Fish, Mouse, Rabbit
Immunogen Species
Rabbit
Immunogen Information
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser276 of perch Cx35 conjugated to KLH
Antigen
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser276 of perch connexin35/36. The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Preparation and Storage
Storage at -20 degree C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20 degree C.
Shipping: Blue Ice
Country of Origin: USA
Shipping: Blue Ice
Country of Origin: USA
Western Blot (WB)
(Western blot of connexin35/36 fusion proteins showing specific immunolabeling of the ~35 kDa connexin-35/36 phosphorylated at Ser276. The first lane (1) is PKA phosphorylated protein purified from the C-Terminal domain. The second lane (2) shows phosphospecificity indicated by no labeling in protein without PKA stimulation. The third lane (3) is protein purified from the intracellular loop, demonstrating that there is no cross-reactivity with the other major PKA phosphorylation site at Ser110.)
Western Blot (WB)
(Western blot of connexin35/36 fusion proteins showing specific immunolabeling of the ~35 kDa connexin-35/36 phosphorylated at Ser276. The first lane (1) is PKA phosphorylated protein purified from the C-Terminal domain. The second lane (2) shows phosphospecificity indicated by no labeling in protein without PKA stimulation. The third lane (3) is protein purified from the intracellular loop, demonstrating that there is no cross-reactivity with the other major PKA phosphorylation site at Ser110.)
Related Product Information for anti-Cx35 antibody
Connexin 35/36 is the most abundant gap junction protein in the central nervous system. Connexin gap junction coupling in the retina has been shown to be regulated by light exposure and neurotransmitter signaling, but more recently has been observed to also depend on direct phosphorylation of connexin (Ouyang et al., 2005, Patel et al., 2006). PKA phosphorylation of Ser110 in the intracellular loop and Ser276 in the C-terminal domain can cause uncoupling of connexin 35 gap junctions (Ouyang et al., 2005). The largest uncoupling effect is seen when both of these sites are phosphorylated, with a much smaller degree of uncoupling observed when only one is phosphorylated (Kothmann et al., 2007). C-terminal domain phosphorylation of connexin 35 at Ser276 is observed in teleost, amphibian, and avian species, with connexin 36 phosphorylation at Ser293 being the mammalian equivalent.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
35kDa
Similar Products
Product Notes
The Cx35 (Catalog #AAA502230) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Connexin35/36 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1000 IHC: 1:500. Researchers should empirically determine the suitability of the Cx35 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Connexin35/36, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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