Rabbit STING Polyclonal Antibody | anti-STING antibody

STING Antibody

Cat. Num. AAA9611204

• Gene Names: TMEM173; ERIS; MITA; MPYS; SAVI; NET23; STING; hMITA; hSTING; STING-beta
• Reactivity: Human, Mouse; Predicted: Pig, Bovine, Horse, Sheep, Rabbit
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: STING; Polyclonal Antibody; STING Antibody; endoplasmic reticulum IFN stimulator; Endoplasmic reticulum interferon stimulator; ERIS; FLJ38577; hMITA; hSTING; Mediator of IRF3 activation; MITA; mitochondrial mediator of IRF3 activation; MPYS; N terminal methionine proline tyrosine serine plasma membrane tetraspanner; NET23; Stimulator of interferon genes; Stimulator of interferon genes protein; TM173_HUMAN; Tmem173; Transmembrane protein 173; anti-STING antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Pig, Bovine, Horse, Sheep, Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: STING Antibody detects endogenous levels of STING.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 379

• Applicable Applications for anti-STING antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human STING.
• Tissue Specificity: Ubiquitously expressed. Expressed in skin endothelial cells, alveolar type 2 pneumocytes, bronchial epithelium and alveolar macrophages.
• Subcellular Location: Endoplasmic Reticulum; Plasma Membrane; Mitochondrion
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of STING in lysates of MCF7?, using STING Antibody.)

Related Product Information for anti-STING antibody

Description: Facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Acts by recognizing and binding cyclic di-GMP (c-di-GMP), a second messenger produced by bacteria, and cyclic GMP-AMP (cGAMP), a messenger produced in response to DNA virus in the cytosol: upon binding of c-di-GMP or cGAMP, autoinhibition is alleviated and TMEM173/STING is able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway. Essential for the induction of IFN-beta in response to human herpes simplex virus 1 (HHV-1) infection. Exhibits 2',3' phosphodiester linkage-specific ligand recognition. Can bind both 2'-3' linked cGAMP and 3'-3' linked cGAMP but is preferentially activated by 2'-3' linked cGAMP (PubMed:26300263).

Post Translational Modifications: Phosphorylated on tyrosine residues upon MHC-II aggregation (By similarity). Phosphorylated on Ser-358 by TBK1, leading to activation and production of IFN-beta. Ubiquitinated (PubMed:19285439, PubMed:19433799, PubMed:21074459, PubMed:25254379). 'Lys-63'-linked ubiquitination mediated by TRIM56 at Lys-150 promotes homodimerization and recruitment of the antiviral kinase TBK1 and subsequent production of IFN-beta (PubMed:21074459). 'Lys-48'-linked polyubiquitination at Lys-150 occurring after viral infection is mediated by RNF5 and leads to proteasomal degradation (PubMed:19285439). 'Lys-11'-linked polyubiquitination at Lys-150 by RNF26 leads to stabilize TMEM173/STING: it protects TMEM173/STING from RNF5-mediated 'Lys-48'-linked polyubiquitination (PubMed:25254379).

Subunit Structure: Associates with the MHC-II complex (By similarity). Homodimer; 'Lys-63'-linked ubiquitination at Lys-150 is required for homodimerization. Interacts with DDX58/RIG-I, MAVS and SSR2. Interacts with RNF5 and TRIM56. Interacts with TBK1; when homodimer, leading to subsequent production of IFN-beta. Interacts with IFIT1 and IFIT2. Interacts with TRIM29; this interaction induces TMEM173/STING ubiquitination and subsequent degradation (PubMed:29038422).

Similarity: The c-di-GMP-binding domain (CBD) forms a homodimer via hydrophobic interactions and binds both the cyclic diguanylate monophosphate (c-di-GMP) and the cyclic GMP-AMP (cGAMP) messengers. In absence of c-di-GMP or cGAMP, the protein is autoinhibited by an intramolecular interaction between the CBD and the C-terminal tail (CTT). Binding of c-di-GMP or cGAMP to the CBD releases the autoinhibition by displacing the CTT, leading to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon. The N-terminal part of the CBD region was initially though to contain a fifth transmembrane region (TM5) but is part of the folded, soluble CBD (PubMed:22579474, PubMed:22705373, PubMed:22728658, PubMed:22728660 and PubMed:22728659). Belongs to the TMEM173 family.

NCBI and Uniprot Product Information

• NCBI GI #: 38093659
• NCBI GeneID: 340061
• NCBI Accession #: NP_938023.1
• NCBI GenBank Nucleotide #: NP_938023.1
• UniProt Accession #: Q86WV6
• Molecular Weight: Observed: 42 kDa; Predicted: 43 kDa
• NCBI Official Full Name: stimulator of interferon genes protein isoform 1
• NCBI Official Synonym Full Names: transmembrane protein 173
• NCBI Official Symbol: TMEM173
• NCBI Official Synonym Symbols: ERIS; MITA; MPYS; SAVI; NET23; STING; hMITA; hSTING; STING-beta
• NCBI Protein Information: stimulator of interferon genes protein
• UniProt Protein Name: Stimulator of interferon genes protein
• UniProt Gene Name: TMEM173
• UniProt Synonym Gene Names: ERIS; MITA; STING; hSTING; ERIS; hMITA
• UniProt Entry Name: STING_HUMAN

NCBI Description

This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]