Rabbit FLIP, cellular Polyclonal Antibody | anti-fliP antibody

FLIP, cellular (cFLIP, FLICE Inhibitory Protein)

Cat. Num. AAA611022

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: FLIP; cellular; Polyclonal Antibody; cellular (cFLIP; FLICE Inhibitory Protein); Anti -FLIP; anti-fliP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This immunoaffinity purified antibody detects 28-58kD proteins, corresponding to the apparent molecular mass of all c-FLIP variants on SDS-PAGE immunoblots, in human, mouse and rat cell lysates and tissue extracts.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 6.7-7.0, containing 0.02% sodium azide.

• Applicable Applications for anti-fliP antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Western Blot: 0.5-1ug/ml, Immunocytochemistry: 5ug/ml, Optimal dilutions to be determined by researcher.
• Immunogen: A 18 residue synthetic peptide based on the amino terminus of human c-FLIP (residues 2-19) was synthesized and the peptide coupled to KLH. This sequence is identical in all c-FLIP splice variants. The amino acid sequence is approximately 90% identical to that of mouse.
• Positive Control: HeLa Cell Lysate
• Preparation and Storage: -20 degree C

Related Product Information for anti-fliP antibody

cFLIP (cellular FLICE-like inhibitory protein) is a cellular homologue of the viral protein, vFLIP, and is also known as CASH, Casper, CLARP, FLAME, I-FLICE, MRIT and Usurpin (2,3). cFLIP contains two death effector domains (DEDs) and is expressed as either a short or long splice variant called cFLIPs and cFLIPL, respectively (3,4). cFLIP can block Fas-mediated apoptosis presumably through the C-terminal region of cFLIPL which has homology with the proteolytic domain of caspase-8 (FLICE). However, cFLIP lacks the active proteinase site required for catalytic activity. Therefore, although it can be recruited to the ligated Fassignalling complex, it cannot deliver a death signal. cFLIP is expressed in the cytosol and it has been reported that cells with high levels of cFLIP relative to FLICE are generally resistant to apoptosis, whereas cells with low levels of cFLIP relative to FLICE are more sensitive to apoptosis (5). cFLIP acts as a caspase-8 dominant negative regulator, blocking apoptosis induced by the oligimerization of the adapter protein, FADD (5). cFLIP interacts with FADD and caspase-8 through their respective DEDs (2). The regulation and role of cFLIP in mediating signals that promote survival such as NF-kB versus inhibiting cell death by blocking cell death receptor-induced caspase activation is unclear (6).

Product Categories/Family for anti-fliP antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 25166005
• NCBI GeneID: 1257484
• UniProt Accession #: Q8D3F1
• Molecular Weight: 29,975 Da
• NCBI Official Full Name: fliP
• NCBI Official Symbol: fliP
• NCBI Protein Information: fliP; flagellar biosynthesis protein FliP
• UniProt Protein Name: FliP protein
• Protein Family: Flagellar biosynthetic protein
• UniProt Gene Name: fliP
• UniProt Entry Name: Q8D3F1_WIGBR

Uniprot Description

Subcellular location: Membrane; Multi-pass membrane protein

By similarity RuleBase RU003852.

Sequence similarities: Belongs to the fliP/mopC/spaP family. RuleBase RU003852