Rabbit FLIP Polyclonal Antibody | anti-CFLAR antibody

FLIP Antibody

Cat. Num. AAA150931

• Gene Names: CFLAR; CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: FLIP Antibody is affinity chromatography purified via peptide column.
• Synonyms: FLIP; Polyclonal Antibody; FLIP Antibody; CASH; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1; CASP8 and FADD-like apoptosis regulator; Caspase homolog; anti-CFLAR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FLIP recognizes all FLIP splice variants including FLIPa, b, and y.
• Purity/Purification: FLIP Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 480

• Applicable Applications for anti-CFLAR antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: FLIP antibody can be used for Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: FLIP antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human FLIP. The sequence is identical in all FLIP splice variants.
• Buffer: FLIP Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: FLIP antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of FLIP in (A) HeLa cell and (B) mouse liver tissue lysate with FLIP antibody at 1 μg/ml.)

Immunohistochemistry (IHC)

(Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 20 μg/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry of FLIP in HeLa cells with FLIP antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of FLIP in mouse liver tissue with FLIP antibody at 20 μg/ml.Red: FLIP Antibody (1159)Blue: DAPI staining)

Related Product Information for anti-CFLAR antibody

FLIP Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.

NCBI and Uniprot Product Information

• NCBI GI #: 2253679
• NCBI GeneID: 8837
• UniProt Accession #: O15519; O14673; O14674; O14675; O15137; O15138; O15356; O15510; O43618; O43619; B4DJE0; B7Z9F9
• Molecular Weight: 44,112 Da
• NCBI Official Full Name: FLICE-like inhibitory protein long form
• NCBI Official Synonym Full Names: CASP8 and FADD-like apoptosis regulator
• NCBI Official Symbol: CFLAR
• NCBI Official Synonym Symbols: CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
• NCBI Protein Information: CASP8 and FADD-like apoptosis regulator; usurpin beta; caspase homolog; inhibitor of FLICE; caspase-eight-related protein; MACH-related inducer of toxicity; FADD-like antiapoptotic molecule 1; caspase-related inducer of apoptosis; cellular FLICE-like inhibitory protein; caspase-like apoptosis regulatory protein
• UniProt Protein Name: CASP8 and FADD-like apoptosis regulator
• Protein Family: Flippase kinase
• UniProt Gene Name: CFLAR
• UniProt Synonym Gene Names: CASH; CASP8AP1; CLARP; MRIT; CASH; Casper; CLARP; c-FLIP; FLAME-1; I-FLICE; MRIT
• UniProt Entry Name: CFLAR_HUMAN

NCBI Description

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]

Uniprot Description

CFLAR: Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. TNFRSF6 stimulation triggers recruitment to the death- inducing signaling complex (DISC) formed by TNFRSF6, FADD and caspase-8. A proteolytic fragment (p43) stays associated with the DISC. Also interacts with caspase-10, caspase-3, TRAF1, TRAF2 and Bcl-X(L) (in vitro). Interacts with HBV protein X. Repressed by IL2/interleukin-2 after TCR stimulation, during progression to the S phase of the cell cycle. Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. Belongs to the peptidase C14A family. 14 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 2q33-q34

Cellular Component: cytoplasm; CD95 death-inducing signaling complex; cytosol; lipid raft

Molecular Function: protein binding; protease binding; cysteine-type endopeptidase activity; enzyme activator activity; death receptor binding; protein complex binding

Biological Process: caspase activation; skeletal muscle development; skeletal muscle atrophy; skeletal myofibril assembly; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; apoptosis; skeletal muscle regeneration; regulation of satellite cell proliferation; proteolysis; activation of NF-kappaB transcription factor; negative regulation of apoptosis