Rabbit FLIP Polyclonal Antibody | anti-FLIP antibody

FLIP (H204) Polyclonal Antibody

Cat. Num. AAA3002762

• Gene Names: CFLAR; CASH; FLIP; MRIT; CLARP; FLAME; cFLIP; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: FLIP; Polyclonal Antibody; FLIP (H204) Polyclonal Antibody; CASP8 and FADD-like apoptosis regulator; Caspase homolog; CASH; Caspase-eight-related protein; Casper; Caspase-like apoptosis regulatory protein; CLARP; Cellular FLICE-like inhibitory protein; c-FLIP; FADD-like antiapoptotic molecule 1; FLAME-1; Inhibitor of FLICE; I-FLICE; MACH-related inducer of toxicity; MRIT; Usurpin; CFLAR; CASP8AP1; anti-FLIP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FLIP (H204) polyclonal antibody detects endogenous levels of FLIP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 480

• Applicable Applications for anti-FLIP antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 171-200 of Human FLIP.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of FLIP (H204) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-FLIP antibody

FLIP (FLICE inhibitory protein) is expressed as both long and short forms and is involved in the regulation of apoptosis. The short form of FLIP contains two death effector domains homologous to the death effector domain of the Fas-associating protein FADD. The long form of FLIP, which shares significant homology with the cysteine protease FLICE, contains an additional caspase-like domain, but lacks a catalytic active site and lacks the residues that form the substrate binding pocket in most caspases. FLIP has been designated by independent groups as Casper, I-FLICE, CLARP, FLAME-1 and MRIT. Although its exact role is still being elucidated, FLIP appears to be an important factor in the regulation of apoptosis downstream of all known death receptors.

NCBI and Uniprot Product Information

• NCBI GI #: 12643547
• NCBI GeneID: 8837
• UniProt Accession #: O15519
• Molecular Weight: ~ 55kDa
• NCBI Official Full Name: CASP8 and FADD-like apoptosis regulator
• NCBI Official Synonym Full Names: CASP8 and FADD like apoptosis regulator
• NCBI Official Symbol: CFLAR
• NCBI Official Synonym Symbols: CASH; FLIP; MRIT; CLARP; FLAME; cFLIP; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
• NCBI Protein Information: CASP8 and FADD-like apoptosis regulator
• UniProt Protein Name: CASP8 and FADD-like apoptosis regulator
• Protein Family: Flippase kinase
• UniProt Gene Name: CFLAR
• UniProt Synonym Gene Names: CASH; CASP8AP1; CLARP; MRIT; CASH; Casper; CLARP; c-FLIP; FLAME-1; I-FLICE; MRIT
• UniProt Entry Name: CFLAR_HUMAN

NCBI Description

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]