Rabbit FLIP L Polyclonal Antibody | anti-FLIP L antibody

RABBIT ANTI HUMAN FLIP L (C-TERMINAL)

Cat. Num. AAA221569

• Gene Names: CFLAR; CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
• Applications: Immunohistochemistry, Western Blot
• Synonyms: FLIP L; Polyclonal Antibody; RABBIT ANTI HUMAN FLIP L (C-TERMINAL); anti-FLIP L antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises the 55kD FLIPL protein, a FLICE inhibitory protein.FLIP was cloned independently in a number of laboratories, and is also known as Casper, I-FLICE, FLAME-1, CASH and CLARP. FLIP has two death effector domains (DEDs) and a caspase-like domain. It interacts with FADD and caspase-8 and -10, potently inhibiting apoptosis by all known death receptors.FLIP exists in a short form (FLIPS) and a long form (FLIPL). Only FLIPL is recognised by this antibody.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5 mg/ml (varies by lot)
• Sequence Length: 480

• Applicable Applications for anti-FLIP L antibody: Immunohistology Frozen, Western Blot (WB)
• Application Notes: Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/2000
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: Peptide at C-terminus of human FLIPL; Buffer Solution
• Antiserum Preparation: Antisera to FLIP were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C for one month or at -20 degree C for longer. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysates from HeLa (H), Jurkat (J) and K562 cell lines probed with Rabbit anti Human FLIP (MBS221569))

Testing Data #2

(Immunocytochemical staining of Jurkat cells with Rabbit anti Human FLIP (MBS221569))

Related Product Information for anti-FLIP L antibody

MBS221569 recognises the 55kD FLIPL protein, a FLICE inhibitory protein. FLIP was cloned independently in a number of laboratories, and is also known as Casper, I-FLICE, FLAME-1, CASH and CLARP. FLIP has two death effector domains (DEDs) and a caspase-like domain. It interacts with FADD and caspase-8 and -10, potently inhibiting apoptosis by all known death receptors. FLIP exists in a short form (FLIPS) and a long form (FLIPL). Only FLIPL is recognised by this antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 187608577
• NCBI GeneID: 8837
• NCBI Accession #: NP_001120655.1
• NCBI GenBank Nucleotide #: NM_001127183.2
• UniProt Accession #: O15519; O14673; O14674; O14675; O15137; O15138; O15356; O15510; O43618; O43619; B4DJE0; B7Z9F9
• Molecular Weight: 44,112 Da
• NCBI Official Full Name: CASP8 and FADD-like apoptosis regulator isoform 1
• NCBI Official Synonym Full Names: CASP8 and FADD-like apoptosis regulator
• NCBI Official Symbol: CFLAR
• NCBI Official Synonym Symbols: CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
• NCBI Protein Information: CASP8 and FADD-like apoptosis regulator; FADD-like antiapoptotic molecule 1; MACH-related inducer of toxicity; caspase homolog; caspase-eight-related protein; caspase-like apoptosis regulatory protein; caspase-related inducer of apoptosis; cellular FLICE-like inhibitory protein; inhibitor of FLICE; usurpin beta
• UniProt Protein Name: CASP8 and FADD-like apoptosis regulator
• UniProt Gene Name: CFLAR
• UniProt Synonym Gene Names: CASH; CASP8AP1; CLARP; MRIT; CASH; Casper; CLARP; c-FLIP; FLAME-1; I-FLICE; MRIT
• UniProt Entry Name: CFLAR_HUMAN

NCBI Description

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]

Uniprot Description

CFLAR: Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. TNFRSF6 stimulation triggers recruitment to the death- inducing signaling complex (DISC) formed by TNFRSF6, FADD and caspase-8. A proteolytic fragment (p43) stays associated with the DISC. Also interacts with caspase-10, caspase-3, TRAF1, TRAF2 and Bcl-X(L) (in vitro). Interacts with HBV protein X. Repressed by IL2/interleukin-2 after TCR stimulation, during progression to the S phase of the cell cycle. Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. Belongs to the peptidase C14A family. 14 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 2q33-q34

Cellular Component: cytoplasm; CD95 death-inducing signaling complex; cytosol; lipid raft

Molecular Function: protein binding; protease binding; cysteine-type endopeptidase activity; enzyme activator activity; death receptor binding; protein complex binding

Biological Process: caspase activation; skeletal muscle development; skeletal myofibril assembly; positive regulation of I-kappaB kinase/NF-kappaB cascade; skeletal muscle atrophy; viral reproduction; apoptosis; skeletal muscle regeneration; regulation of satellite cell proliferation; proteolysis; negative regulation of apoptosis; activation of NF-kappaB transcription factor