Human 26S Proteasome Non-ATPase Regulatory Subunit 3 (PSMD3) RTU ELISA Kit | PSMD3 rtu elisa kit

Human 26S Proteasome Non-ATPase Regulatory Subunit 3 (PSMD3) ELISA Kit

Cat. Num. AAA4503738

• Gene Names: PSMD3; S3; P58; RPN3; TSTA2
• Reactivity: Human
• Synonyms: 26S Proteasome Non-ATPase Regulatory Subunit 3 (PSMD3); Human 26S Proteasome Non-ATPase Regulatory Subunit 3 (PSMD3) ELISA Kit; 26S Proteasome Regulatory Subunit RPN3; 26S Proteasome Regulatory Subunit S3; Proteasome Subunit p58; PSMD3 rtu elisa kit

• Reactivity: Human
• Sequence Length: 534

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.104ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSMD3 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PSMD3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PSMD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PSMD3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PSMD3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 25777612
• NCBI GeneID: 5709
• NCBI Accession #: NP_002800.2
• NCBI GenBank Nucleotide #: NM_002809.3
• UniProt Accession #: O43242; Q96EI2; Q9BQA4; B3KMW9; B4DT72
• Molecular Weight: 41,184 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 3
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 3
• NCBI Official Symbol: PSMD3
• NCBI Official Synonym Symbols: S3; P58; RPN3; TSTA2
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 3
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 3
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD3

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S3 family that functions as one of the non-ATPase subunits of the 19S regulator lid. Single nucleotide polymorphisms in this gene are associated with neutrophil count. [provided by RefSeq, Jul 2012]

Uniprot Description

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.