Western Blot (WB)
(Fig.1. Western blot analysis of 1) HepG2, 2) 293T, 3) mouse brain tissue, 4) rat brain tissue, diluted at 1:5000.)
Mouse Lamin B1 Monoclonal Antibody | anti-B1 antibody
Anti-Lamin B1 Monoclonal Antibody (15T1)
IHC-P: 1:50-1:300
IF: 1:200
IP: 1:200
Shipping||Gel pack with blue ice.
Western Blot (WB)
(Fig.1. Western blot analysis of 1) HepG2, 2) 293T, 3) mouse brain tissue, 4) rat brain tissue, diluted at 1:5000.)
Western Blot (WB)
(Fig.1. Western blot analysis of 1) HepG2, 2) 293T, 3) mouse brain tissue, 4) rat brain tissue, diluted at 1:5000.)
Immunofluorescence (IF)
(Fig.2 Immunofluorescence analysis of human lung cancer tissue. 1, Lamin B1 Monoclonal Antibody (15T1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunofluorescence (IF)
(Fig.2 Immunofluorescence analysis of human lung cancer tissue. 1, Lamin B1 Monoclonal Antibody (15T1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunohistochemistry (IHC)
(Fig.3. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, Lamin B1 Monoclonal Antibody (15T1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Fig.3. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, Lamin B1 Monoclonal Antibody (15T1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Background: LMNB1 encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of LMNB1 is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI Description
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010]
Uniprot Description
Lamin B1: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Nuclear lamins are cleaved by caspases during apoptosis.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 5q23.2
Cellular Component: nucleoplasm; nuclear membrane; membrane; lamin filament; nuclear inner membrane; nuclear envelope
Molecular Function: phospholipase binding; structural molecule activity
Biological Process: apoptosis; cell structure disassembly during apoptosis
Disease: Leukodystrophy, Demyelinating, Adult-onset, Autosomal Dominant
Research Articles on B1
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Product Notes
The B1 lmnb1 (Catalog #AAA9719117) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Lamin B1 Monoclonal Antibody (15T1) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Lamin B1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:2000-1:5000 IHC-P: 1:50-1:300 IF: 1:200 IP: 1:200. Researchers should empirically determine the suitability of the B1 lmnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lamin B1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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