Rabbit anti-Rat, Mouse DISC1 Polyclonal Antibody | anti-DISC1 antibody

DISC1 (a.a. 740-753) Antibody

Cat. Num. AAA474301

• Reactivity: Rat, Mouse
• Applications: Western Blot, ELISA
• Synonyms: DISC1; Polyclonal Antibody; DISC1 (a.a. 740-753) Antibody; DISC1 (a.a. 740-753); DISC1 B; anti-DISC1 antibody

• Host: Rabbit
• Reactivity: Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified using DISC1 (a.a. 740-753) peptide (without carrier). The antibody detects a 100kDa* protein corresponding to the molecular mass of DISC1 on SDS-PAGE immunoblots of mouse brain and rat PC12 cells.
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-DISC1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: 1:2000; WB: 1:1000
• Immunogen: DISC1 synthetic peptide (coupled to KLH) containing amino acid residues 740 to 753 from mouse DISC1. This peptide sequence is well conserved in rat DISC1, but has 7 amino acid differences with the conserved site in human DISC1.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot of DISC1 in mouse brain (lanes 1 & 3) and rat PC12 cells (lanes 2 & 4). The blots were probed with DP3021 anti-DISC1 (a.a. 740-753) (lanes 1 & 2) and DP3041 anti-DISC1 (a.a. 740-753) (lanes 3 & 4).)

Related Product Information for anti-DISC1 antibody

Disrupted in schizophrenia 1 (DISC1) is a multifunctional scaffold protein that has important roles in neurodevelopment. DISC1 is expressed in both neuronal progenitor cells and postmitotic neurons in the developing cerebral cortex. DISC1 can interact with both centrosomal proteins and dynein-motor related proteins. In addition, DISC1 interacts with and inhibits the kinase GSK-3beta to enable Wnt activation of beta-catenin-mediated gene transcription and neuron proliferation. These functions of DISC1 may be regulated by post-translational modification. PKA and CDK5 can phosphorylate Ser-710, and a non-phosphorylatable Ser-710 mutant shows decreased interaction with the centrosomal proteins, BBS1 and BBS4, while a constituitively phosphorylated Ser-710 mutant shows increased interaction with these proteins. During neuronal progenitor cell proliferation, DISC1 phosphorylation at Ser-710 is low and the interaction with GSK-3beta is enhanced. By contrast, DISC1 phosphorylation is increased, association with GSK-3beta is decreased, and interaction with BBS1 is enhanced during postmitotic neuron migration.

References

Brandon, N.J. et al. (2011) Nat Rev Neurosci. 12(12):707.
Ishizuka, K. (2011) Nature. 473(7345):92.
Mao, Y. et al. (2009) Cell. 136(6):1017.

NCBI and Uniprot Product Information

• NCBI GI #: 119874423
• NCBI GeneID: 244667
• NCBI Accession #: NP_777278.2
• NCBI GenBank Nucleotide #: NM_174853.2
• UniProt Accession #: Q811T9
• Molecular Weight: 100 kDa
• NCBI Official Full Name: disrupted in schizophrenia 1 homolog isoform 2
• NCBI Official Synonym Full Names: disrupted in schizophrenia 1
• NCBI Official Symbol: Disc1
• NCBI Protein Information: disrupted in schizophrenia 1 homolog
• UniProt Protein Name: Disrupted in schizophrenia 1 homolog
• Protein Family: Disrupted in schizophrenia
• UniProt Gene Name: Disc1
• UniProt Entry Name: DISC1_MOUSE