Mouse Sphingosine Kinase 2 Monoclonal Antibody | anti-SPHK2 antibody

Sphingosine Kinase 2 (N-terminal region) M532 Antibody

Cat. Num. AAA474593

• Gene Names: SPHK2; SK 2; SK-2; SPK 2; SPK-2
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA
• Synonyms: Sphingosine Kinase 2; Monoclonal Antibody; Sphingosine Kinase 2 (N-terminal region) M532 Antibody; Sphingosine Kinase 2 (N-terminal region); anti-SPHK2 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M532
• Specificity: This antibody was purified using Protein G affinity purification. The antibody detects 70kDa* proteins corresponding to the molecular mass of SK2 on SDS-PAGE immunoblots of human recombinant SK2 and endogenous SK2 in human HeLa cells.
• Form/Format: Mouse monoclonal, protein G purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-SPHK2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: 1:1000; WB: 1:250
• Immunogen: Clone M532 was generated from a SK2 (N-terminal region) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human SK2. This peptide sequence is highly conserved in rat and mouse SK2 proteins, and has no homology to SK1.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot of human recombinant SK2 (lanes 1-4). The blots were probed with rabbit polyclonal anti-SK2 (N-terminal region) at 1:250 (lane 1) and 1:1000 (lane 2) or with mouse monoclonal anti-SK2 (N-terminal region) at 1:250 (lane 3) or 1:1000 (lane 4).)

Related Product Information for anti-SPHK2 antibody

Sphingolipids are metabolized into bioactive products that include ceramide, sphingosine, and sphingosine-1-phosphate (S1P). Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA for both of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen and lung, while SK2 is highest in kidney and liver. SKs can be activated through growth factor, G protein-coupled, and immunoglobulin receptor signalling. Regulation of SK1 and SK2 activity may occur through phosphorylation. SK1 is phosphorylated at Ser-225 by ERK leading to increased activity and translocation to the plasma membrane. SK2 is phosphorylated in response to EGF, PKC activators, and phorbol esters. ERK1 can phosphorylate both Ser-351 and Thr-578, and non-phosphorylatable mutants of these sites suppress ERK1-mediated chemotaxis.

References

Hait, N.C. et al. (2007) J Biol. Chem. 282(16):12058.Hait, N.C. et al. (2005) J Biol. Chem. 280:29462.Spiegel S. & Milstien S. (2003) Nat Rev Mol Cell Biol. 4:397.

NCBI and Uniprot Product Information

• NCBI GI #: 323462187
• NCBI GeneID: 56848
• NCBI Accession #: NP_001191088.1
• NCBI GenBank Nucleotide #: NM_001204159.2
• UniProt Accession #: Q9NRA0; Q9BRN1; Q9H0Q2; Q9NWU7
• Molecular Weight: 69,217 Da
• NCBI Official Full Name: sphingosine kinase 2 isoform a
• NCBI Official Synonym Full Names: sphingosine kinase 2
• NCBI Official Symbol: SPHK2
• NCBI Official Synonym Symbols: SK 2; SK-2; SPK 2; SPK-2
• NCBI Protein Information: sphingosine kinase 2; sphingosine kinase type 2
• UniProt Protein Name: Sphingosine kinase 2
• Protein Family: Sphingosine kinase
• UniProt Gene Name: SPHK2
• UniProt Synonym Gene Names: SK 2; SPK 2
• UniProt Entry Name: SPHK2_HUMAN

NCBI Description

This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

SPHK2: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro- dihydrosphingosine, D-erythro-sphingosine and L-threo- dihydrosphingosine. Binds phosphoinositides. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - sphingolipid; EC 2.7.1.91; Kinase, lipid

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: membrane; lysosomal membrane; cytosol

Molecular Function: D-erythro-sphingosine kinase activity; protein binding; Ras GTPase binding; sphinganine kinase activity; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: blood vessel development; cell proliferation; sphingolipid metabolic process; sphingolipid biosynthetic process; positive regulation of cell proliferation; protein kinase C activation; sphingosine metabolic process; sphinganine-1-phosphate biosynthetic process; brain development; lipid phosphorylation; negative regulation of apoptosis