Rabbit Vav2 Polyclonal Antibody | anti-Vav2 antibody

Vav2 (Tyr-172)[conserved site], phospho-specific Antibody

Cat. Num. AAA474644

• Gene Names: Vav2; Vav-2; AI847175; 2810040F13Rik
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA, Immunocytochemistry
• Synonyms: Vav2; Polyclonal Antibody; Vav2 (Tyr-172)[conserved site]; phospho-specific Antibody; phospho-specific; anti-Vav2 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody was cross-adsorbed to unphosphorylated Vav2 (Tyr-172) peptide before affinity purification using phospho-Vav2 (Tyr-172) peptide (without carrier). The antibody detects a 100kDa* band corresponding to Vav on SDS-PAGE immunoblots of human A431 cells treated with EGF and detects Vav1, Vav2, and Vav3 in Jurkat cells treated with pervanadate. The antibody does not detect these Vavs after treatment with alkaline phosphatase.
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-Vav2 antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:2000; ICC: 1:100; WB: 1:1000
• Immunogen: Phospho-Vav2 (Tyr-172) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-172 in mouse Vav2. This sequence has high homology with similar regions in rat and human Vav2, and has significant homology to the conserved site in Vav1 and Vav3.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot of human A431 cells treated with EGF (lanes 1, 3, & 5) then the blot was exposed to alkaline phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Vav (a.a. 165-174) (lanes 1 & 2), anti-Vav2 (Tyr-142) (lanes 3 & 4), or anti-Vav2 (Tyr-172) (lanes 5 & 6))

Immunocytochemistry (ICC)

(Immunocytochemical labeling of VAV2 in control and pervanadate-treated human A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40. Then labeled with rabbit polyclonal Vav2 (Tyr-172). The antibody was detected using goat anti-rabbit DyLight 594.)

Related Product Information for anti-Vav2 antibody

The Vav family of Rho-guanine nucleotide exchange factors, Vav1, Vav2, and Vav3, have central roles in transducing signals from cell surface receptors, such as integrin, growth factor and immune cell receptors to the cytoskeleton. This role includes receptor-mediated changes in the actin cytoskeleton and cell motility. Vav1 expression is normally restricted to hematopoietic cells, while Vav2 and Vav3 are more widely expressed. All three Vav isoforms have been shown to be abnormally expressed in several types of cancer. Vavs are composed of multiple domains, including a Dbl homology domain, a calponin homology domain, an acidic amino acid region, a pleckstrin homology domain, a cysteine-rich domain, and SH3 and SH2 domains. Vav activity is regulated by the phosphorylation status of several conserved tyrosine residues in the acidic region (In Vav2: Tyr-142, Tyr-159, and Tyr-172). These tyrosine residues are able to participate in autoinhibitory interactions with the Dbl homology domain and this interaction is prevented after phosphorylation of these sites leading to activation of Vav GEF activity.

References

Wilsbacher, J.L. et al. (2006) Cell Comm. Signal. 4:5.
Aghazadeh, B. (2000) Cell 102:625.
Schuebel, K.E. et al. (1996) Oncogene 13:363.

NCBI and Uniprot Product Information

• NCBI GI #: 1353412
• NCBI GeneID: 22325
• UniProt Accession #: Q60992; A2AH49
• Molecular Weight: 101.3
• NCBI Official Full Name: Vav2
• NCBI Official Synonym Full Names: vav 2 oncogene
• NCBI Official Symbol: Vav2
• NCBI Official Synonym Symbols: Vav-2; AI847175; 2810040F13Rik
• NCBI Protein Information: guanine nucleotide exchange factor VAV2
• UniProt Protein Name: Guanine nucleotide exchange factor VAV2
• Protein Family: Guanine nucleotide exchange factor
• UniProt Gene Name: Vav2
• UniProt Synonym Gene Names: VAV-2
• UniProt Entry Name: VAV2_MOUSE

NCBI Description

This gene encodes a member of the Vav family of Rho guanine nucleotide exchange factors. Vav family proteins are involved in the development and activation of lymphocytes, and the encoded protein may also be involved in angiogenesis. Disruption of this gene in mice is associated with heart, artery, and kidney defects, as well as tachycardia and hypertension. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]