Western Blot (WB)
(Western blot image of rat PC12 cells (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 (lanes 2 & 4). The blot was probed with rabbit polyclonals anti-TRPM7 (Extracellular region) TP5651 (lanes 1 & 2) or anti-TRPM7 (Ser-1569) phospho-specific (lanes 3 & 4).)
Rabbit TRPM7 Polyclonal Antibody | anti-TRPM7 antibody
TRPM7 (Extracellular region) Antibody
Gene Names
TRPM7; CHAK; CHAK1; ALSPDC; LTRPC7; TRP-PLIK
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Synonyms
TRPM7; Polyclonal Antibody; TRPM7 (Extracellular region) Antibody; TRPM7 (Extracellular region); TRPM7 1017pAb; anti-TRPM7 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
The antibody was affinity purified using TRPM7 (Extracellular region) peptide. This antibody antibody detects a 220kDa* protein on SDS-PAGE immunoblots of human MCF7, MDA-MB-231, mouse brain, and rat PC12 cells.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-TRPM7 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1:2000
WB: 1:500
WB: 1:500
Immunogen
TRPM7 synthetic peptide (coupled to carrier) corresponding to amino acids in the extracellular region of human TRPM7. This site is well conserved in rat and mouse TRPM7, but has low homology to other TRPM family members.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot image of rat PC12 cells (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 (lanes 2 & 4). The blot was probed with rabbit polyclonals anti-TRPM7 (Extracellular region) TP5651 (lanes 1 & 2) or anti-TRPM7 (Ser-1569) phospho-specific (lanes 3 & 4).)
Western Blot (WB)
(Western blot image of rat PC12 cells (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 (lanes 2 & 4). The blot was probed with rabbit polyclonals anti-TRPM7 (Extracellular region) TP5651 (lanes 1 & 2) or anti-TRPM7 (Ser-1569) phospho-specific (lanes 3 & 4).)
Related Product Information for anti-TRPM7 antibody
The transient receptor potential melastatin (TRPM) subfamily of cation-permeable TRP channels is ubiquitously expressed in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, TRPM6 and TRPM7 possess an inherent serine/threonine kinase activity. TRPM7 specifically is involved with cellular magnesium homeostasis and neurotransmitter release. Due to the magnesium inhibition, TRPM7's ion channel activity is very low. TRPM7 has been implicated in cell proliferation and migration during cancer progression, and its expression levels correlate with prognosis in breast cancer. TRPM7 kinase activation leads to massive autophosphorylation of the C-terminal region, including phosphorylation of Ser-1493, Ser-1513, and Ser-1569. Both Ser-1513 and Ser-1569 phosphorylation is required for kinase activity, and phosphorylation of Ser-1513 may inhibit Caspase-mediated cleavage of the C-terminal tail. Thus, TRPM7 is a multifunctional transmembrane protein with roles in cell signaling, proliferation, migration, and death.
References
Park, HS, et al. (2014) Korean J Phys Pharm. 18(1):15-23.
Desai, BN. et al. (2012) Dev. Cell. 22(6): 1149
Clark, K. et al. (2008) PLoS ONE 3(3): e1876
Masayuki, M. et al. (2005) J of Bio Chem 280(21): 20793
Desai, BN. et al. (2012) Dev. Cell. 22(6): 1149
Clark, K. et al. (2008) PLoS ONE 3(3): e1876
Masayuki, M. et al. (2005) J of Bio Chem 280(21): 20793
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
212,697 Da
NCBI Official Full Name
transient receptor potential cation channel subfamily M member 7
NCBI Official Synonym Full Names
transient receptor potential cation channel, subfamily M, member 7
NCBI Official Symbol
TRPM7
NCBI Official Synonym Symbols
CHAK; CHAK1; ALSPDC; LTRPC7; TRP-PLIK
NCBI Protein Information
transient receptor potential cation channel subfamily M member 7; LTrpC-7; channel-kinase 1; LTRPC ion channel family member 7; long transient receptor potential channel 7; transient receptor potential-phospholipase C-interacting kinase
UniProt Protein Name
Transient receptor potential cation channel subfamily M member 7
Protein Family
UniProt Gene Name
TRPM7
UniProt Synonym Gene Names
CHAK1; LTRPC7; LTrpC-7; LTrpC7
UniProt Entry Name
TRPM7_HUMAN
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Product Notes
The TRPM7 trpm7 (Catalog #AAA474627) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRPM7 (Extracellular region) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TRPM7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:2000 WB: 1:500. Researchers should empirically determine the suitability of the TRPM7 trpm7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRPM7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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