Rabbit alpha6-Tubulin Polyclonal Antibody | anti-TUBA1C antibody

alpha6-Tubulin (a.a. 160-169) Antibody

Cat. Num. AAA474626

• Gene Names: TUBA1C; TUBA6; bcm948
• Reactivity: Human, Rat, Mouse, Chicken, Fish
• Applications: Western Blot, ELISA
• Synonyms: alpha6-Tubulin; Polyclonal Antibody; alpha6-Tubulin (a.a. 160-169) Antibody; alpha6-Tubulin (a.a. 160-169); Tubulina 160; anti-TUBA1C antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Chicken, Fish
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified using alpha6-Tubulin (a.a. 160-169) peptide (without carrier). The antibody detects a 50kDa* protein corresponding to the molecular mass of alpha-Tubulin on SDS-PAGE immunoblots of human smooth muscle cells (A7r5) and mouse brain, as well as human recombinant a1-tubulin.
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-TUBA1C antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: 1:2000; WB: 1:1000
• Immunogen: alpha6-Tubulin (a.a. 160-169) synthetic peptide (coupled to KLH) corresponds to amino acid residues in human alpha6-Tubulin. This sequence is well conserved in most alpha-tubulin isoforms from most eukaryotic species, but is not conserved in beta-Tubulin.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot analysis of 250 ng/lane of a1-Tubulin unphosphorylated (lanes 1, 3, & 5) or phosphorylated at Ser-165 with PKCalpha (lanes 2, 4, & 6). The blots were probed with anti-alpha6-Tubulin (a.a. 160-169) (TP4281; lanes 1 & 2), anti-alpha6-Tubulin (Ser-165) (TP4131; lanes 3 & 4), and anti-alpha-Tubulin (C-terminus) (TM4111; lanes 5 & 6).)

Related Product Information for anti-TUBA1C antibody

Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of a/beta-Tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in alpha- and beta-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of betaIII-Tubulin in vitro, and unphosphorylated Ser-444 may be an early marker for cells of neuronal lineage. Cdk1 can phosphorylate Ser-172 in beta-Tubulin during mitosis and this may impair tubulin incorporation into microtubules. In alpha-tubulin, PKC can phosphorylate Ser-165 leading to increased cell motility in human breast cells.

References

Abeyweera, T.P. et al. (2009) J Biol Chem. 284(26):17648. (space)
Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.
Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.
Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.

NCBI and Uniprot Product Information

• NCBI GI #: 142375723
• NCBI GeneID: 84790
• NCBI Accession #: NP_116093.1
• NCBI GenBank Nucleotide #: NM_032704.3
• UniProt Accession #: Q9BQE3
• Molecular Weight: 49,895 Da
• NCBI Official Full Name: Homo sapiens tubulin, alpha 1c (TUBA1C), mRNA
• NCBI Official Synonym Full Names: tubulin, alpha 1c
• NCBI Official Symbol: TUBA1C
• NCBI Official Synonym Symbols: TUBA6; bcm948
• NCBI Protein Information: tubulin alpha-1C chain; alpha-tubulin 6; tubulin alpha-6 chain; tubulin, alpha 6
• UniProt Protein Name: Tubulin alpha-1C chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBA1C
• UniProt Synonym Gene Names: TUBA6
• UniProt Entry Name: TBA1C_HUMAN

Uniprot Description

Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Subunit structure: Dimer of alpha and beta chains.

Subcellular location: Cytoplasm › cytoskeleton.

Post-translational modification: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively

By similarity.Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules

Probable.Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling

By similarity.

Sequence similarities: Belongs to the tubulin family.