Rabbit anti-Human (Weak) DELTA-LIKE PROTEIN 3 Polyclonal Antibody | anti-DLL3 antibody

RABBIT ANTI MOUSE DELTA-LIKE PROTEIN 3

Cat. Num. AAA224287

• Gene Names: DLL3; SCDO1
• Reactivity: Human (Weak)
• Applications: ELISA, Western Blot
• Synonyms: DELTA-LIKE PROTEIN 3; Polyclonal Antibody; RABBIT ANTI MOUSE DELTA-LIKE PROTEIN 3; anti-DLL3 antibody

• Host: Rabbit
• Reactivity: Human (Weak)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognises mouse Delta-like protein 3 (DLL3), one of the five major ligands of the Notch signalling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4. ; ; The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. ; ; Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. ; ; DLL3 is predominantly expressed in the neuroectoderm and paraxial mesoderm during embryogenesis, where it plays a role in somite segmentation, during early development. DLL3 acts as a regulator of Notch 1, as an inhibitor of primary neurogenesis, and studies in mice have linked DLL3-Notch signalling with liver regeneration, and developmental defects. More than 25 mutations in the DLL3 gene are known to be responsible for the autosomal recessive skeletal malformation condition known as spondylocostal dysostosis type 1 (SCDO1), associated with vertebral and rib segmentation defects.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 618

• Applicable Applications for anti-DLL3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a prominent band of approximately 60-62kDa in mouse brain tissue, and weakly in human brain tissue.; Western Blotting: Minimum Dilution: 1/100; Maximum Dilution: 1/500
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: Keyhole Limpet Hemocyanin (KLH) conjugated peptide sequence CSPEHGYCEEPDE, corresponding to amino acids 222-234 of mouse DLL3.; Buffer Solution
• Antiserum Preparation: Antiserum to mouse DLL3 was raised by repeated immunisation of rabbits with protein peptide sequence. Purified IgG was prepared by affinity chromatography on Protein A.
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Mouse brain lysate probed with Rabbit anti Mouse Delta-Like Protein 3 (MBS224287) followed by Sheep anti Rabbit IgG:HRP (MBS235194))

Related Product Information for anti-DLL3 antibody

This antibody recognises mouse Delta-like protein 3 (DLL3), one of the five major ligands of the Notch signalling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. DLL3 is predominantly expressed in the neuroectoderm and paraxial mesoderm during embryogenesis, where it plays a role in somite segmentation, during early development. DLL3 acts as a regulator of Notch 1, as an inhibitor of primary neurogenesis, and studies in mice have linked DLL3-Notch signalling with liver regeneration, and developmental defects. More than 25 mutations in the DLL3 gene are known to be responsible for the autosomal recessive skeletal malformation condition known as spondylocostal dysostosis type 1 (SCDO1), associated with vertebral and rib segmentation defects.

NCBI and Uniprot Product Information

• NCBI GI #: 8393264
• NCBI GeneID: 10683
• NCBI Accession #: NP_058637.1
• NCBI GenBank Nucleotide #: NM_016941.3
• Molecular Weight: 61,178 Da
• NCBI Official Full Name: delta-like protein 3 isoform 1
• NCBI Official Synonym Full Names: delta-like 3 (Drosophila)
• NCBI Official Symbol: DLL3
• NCBI Official Synonym Symbols: SCDO1
• NCBI Protein Information: delta-like protein 3; delta3; drosophila Delta homolog 3
• UniProt Protein Name: Delta-like protein 3
• Protein Family: Delta-like protein
• UniProt Gene Name: DLL3
• UniProt Synonym Gene Names: Delta3
• UniProt Entry Name: DLL3_HUMAN

NCBI Description

This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DLL3: Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. Can bind and activate Notch-1 or another Notch receptor.

Protein type: Cell development/differentiation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: integral to membrane

Molecular Function: Notch binding

Biological Process: paraxial mesoderm development; compartment specification; somitogenesis; Notch signaling pathway; skeletal development; negative regulation of neurogenesis

Disease: Spondylocostal Dysostosis 1, Autosomal Recessive