Rabbit DELTA-LIKE PROTEIN 1 Polyclonal Antibody | anti-Dll1 antibody

RABBIT ANTI DELTA-LIKE PROTEIN 1

Cat. Num. AAA223595

• Gene Names: Dll1; Delta1
• Reactivity: Chicken, Human, Rat
• Applications: Immunohistochemistry
• Synonyms: DELTA-LIKE PROTEIN 1; Polyclonal Antibody; RABBIT ANTI DELTA-LIKE PROTEIN 1; anti-Dll1 antibody

• Host: Rabbit
• Reactivity: Chicken, Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises Delta-like protein 1 (DLL1), one of the five major ligands of the Notch signaling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4. ; ; The Notch signaling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T-cell development, and both tumor progression and suppression. ; ; Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta-like protein 1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and the gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signaling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. ; ; DLL1 is widely expressed, and acts as a mediator of cell fate decisions during hematopoiesis, and may play a role in cell-to-cell communication in mammalian embryos. DLL1 plays an important role in B and T cell differentiation, in embryonic somite formation and patterning, and associates with the scaffolding protein MAGI1 at adherens junctions on neuronal processes. Signaling through DLL1 and Notch 2 has been implicated in the development of marginal zone B cells (MZB). ; ; This item has been reported to work in ELISA.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 722

• Applicable Applications for anti-Dll1 antibody: Immunohistology Paraffin*
• Application Notes: Immunohistology - Paraffin: Maximum Dilution: 10ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Perservative Stabilisers: 0.01% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide sequence corresponding amino acids 155-173 of Mouse Delta-like protein 1.; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to Human DLL1 were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of formalin fixed, paraffin embedded human brain with Rabbit anti Human DLL1 (MBS223595))

Related Product Information for anti-Dll1 antibody

MBS223595 specifically recognises Delta-like protein 1 (DLL1), one of the five major ligands of the Notch signaling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4. The Notch signaling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta-like protein 1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and the gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signaling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. DLL1 is widely expressed, and acts as a mediator of cell fate decisions during hematopoiesis, and may play a role in cell-to-cell communication in mammalian embryos. DLL1 plays an important role in B and T cell differentiation, in embryonic somite formation and patterning, and associates with the scaffolding protein MAGI1 at adherens junctions on neuronal processes. Signaling through DLL1 and Notch 2 has been implicated in the development of marginal zone B cells (MZB). MBS223595 has been reported to work in ELISA.

NCBI and Uniprot Product Information

• NCBI GI #: 40789272
• NCBI GeneID: 13388
• NCBI Accession #: NP_031891.2
• NCBI GenBank Nucleotide #: NM_007865.3
• UniProt Accession #: Q61483; Q6PFV7
• Molecular Weight: 78,449 Da
• NCBI Official Full Name: delta-like protein 1
• NCBI Official Synonym Full Names: delta-like 1 (Drosophila)
• NCBI Official Symbol: Dll1
• NCBI Official Synonym Symbols: Delta1
• NCBI Protein Information: delta-like protein 1; delta like-1; drosophila Delta homolog 1
• UniProt Protein Name: Delta-like protein 1
• Protein Family: Delta-like protein
• UniProt Gene Name: Dll1
• UniProt Synonym Gene Names: Delta1
• UniProt Entry Name: DLL1_MOUSE

Uniprot Description

DLL1: Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. Interacts with Notch receptors. Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.

Protein type: Cell development/differentiation; Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: membrane; plasma membrane; integral to membrane; cytoplasmic vesicle

Molecular Function: protein binding; calcium ion binding; Notch binding

Biological Process: nervous system development; somitogenesis; Notch signaling pathway; inner ear morphogenesis; negative regulation of myeloid cell differentiation; negative regulation of interleukin-10 production; in utero embryonic development; multicellular organismal development; auditory receptor cell differentiation; odontogenesis of dentine-containing teeth; compartment specification; negative regulation of cell differentiation; somite specification; organ morphogenesis; negative regulation of neuron differentiation; regulation of neurogenesis; cell-cell signaling; negative regulation of auditory receptor cell differentiation; positive regulation of transcription from RNA polymerase II promoter; cell communication; heart looping; determination of left/right symmetry; cell differentiation; auditory receptor cell fate commitment; inner ear development; positive regulation of Notch signaling pathway