Goat anti-Mouse, Rat DELTA-LIKE PROTEIN 1 Polyclonal Antibody | anti-DLL1 antibody

GOAT ANTI DELTA-LIKE PROTEIN 1

Cat. Num. AAA224312

• Gene Names: DLL1; DL1; Delta; DELTA1
• Reactivity: Mouse, Rat
• Applications: ELISA, Western Blot
• Synonyms: DELTA-LIKE PROTEIN 1; Polyclonal Antibody; GOAT ANTI DELTA-LIKE PROTEIN 1; anti-DLL1 antibody

• Host: Goat
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises Delta-like protein 1 (DLL1), one of the five major ligands of the Notch signalling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4. ; ; The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. ; ; Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. ; ; DLL1 is widely expressed, and acts as a mediator of cell fate decisions during haematopoiesis, and may play a role in cell-to-cell communication in mammalian embryos. DLL1 plays an important role in B and T cell differentiation, in embryonic somite formation and patterning, and associates with the scaffolding protein MAGI1 at adherens junctions on neuronal processes. Signalling through DLL1 and Notch 2 has been implicated in the development of marginal zone B cells (MZB).
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 723

• Applicable Applications for anti-DLL1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 75kDa in mouse and rat heart cell lysates.; ELISA: Minimum Dilution: 1/16,000; Western Blotting: Minimum Dilution: 0.3; Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin
• Immunogen: Synthetic peptide sequence C-ATQRHLTVGEEWSQD from the internal region of DLL1 (NP_005609.3).; Buffer Solution
• Antiserum Preparation: Antiserum to human DLL1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Rat Heart cell lysate probed with Goat anti Delta-like protein 1 (MBS224312))

Related Product Information for anti-DLL1 antibody

MBS224312 specifically recognises Delta-like protein 1 (DLL1), one of the five major ligands of the Notch signalling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4.The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation.DLL1 is widely expressed, and acts as a mediator of cell fate decisions during haematopoiesis, and may play a role in cell-to-cell communication in mammalian embryos. DLL1 plays an important role in B and T cell differentiation, in embryonic somite formation and patterning, and associates with the scaffolding protein MAGI1 at adherens junctions on neuronal processes. Signalling through DLL1 and Notch 2 has been implicated in the development of marginal zone B cells (MZB).

NCBI and Uniprot Product Information

• NCBI GI #: 110735443
• NCBI GeneID: 28514
• NCBI Accession #: NP_005609.3
• NCBI GenBank Nucleotide #: NM_005618.3
• Molecular Weight: 27,355 Da
• NCBI Official Full Name: delta-like protein 1
• NCBI Official Synonym Full Names: delta-like 1 (Drosophila)
• NCBI Official Symbol: DLL1
• NCBI Official Synonym Symbols: DL1; Delta; DELTA1
• NCBI Protein Information: delta-like protein 1; H-Delta-1; drosophila Delta homolog 1
• UniProt Protein Name: Delta-like protein 1
• Protein Family: Delta-like protein
• UniProt Gene Name: DLL1
• UniProt Synonym Gene Names: Delta1; H-Delta-1
• UniProt Entry Name: DLL1_HUMAN

NCBI Description

DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. [provided by RefSeq, Jul 2008]

Uniprot Description

DLL1: Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. Interacts with Notch receptors. Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell development/differentiation

Chromosomal Location of Human Ortholog: 6q27

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane; cytoplasmic vesicle

Molecular Function: protein binding; calcium ion binding; Notch binding

Biological Process: regulation of cell adhesion; Notch signaling pathway; negative regulation of myeloid cell differentiation; negative regulation of interleukin-10 production; Notch receptor processing; cell fate determination; compartment specification; somite specification; cell-cell signaling; negative regulation of auditory receptor cell differentiation; positive regulation of transcription from RNA polymerase II promoter; hemopoiesis; heart looping; determination of left/right symmetry; cell differentiation; inner ear development; positive regulation of Notch signaling pathway