Human Multidrug resistance-associated protein 4 ELISA Kit | ABCC4 elisa kit

Human Multidrug resistance-associated protein 4 ELISA Kit

Cat. Num. AAA764500

• Gene Names: ABCC4; MRP4; MOATB; MOAT-B
• Reactivity: Human
• Synonyms: Multidrug resistance-associated protein 4; Human Multidrug resistance-associated protein 4 ELISA Kit; ABCC4 (Multidrug resistance-associated protein 4)/MRP4/MOAT-B/Multi-specific organic anion transporter B/ATP-binding cassette sub-family C member 4/MRP/cMOAT-related ABC transporter; ABCC4 elisa kit

• Reactivity: Human
• Sequence Length: 1278

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.56-100ng/ml
• Sensitivity: 0.938ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ABCC4 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ABCC4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ABCC4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ABCC4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ABCC4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 685504974
• NCBI GeneID: 10257
• NCBI Accession #: NP_001288758.1
• NCBI GenBank Nucleotide #: NM_001301829.1
• Molecular Weight: 88,138 Da
• NCBI Official Full Name: multidrug resistance-associated protein 4 isoform 3
• NCBI Official Synonym Full Names: ATP binding cassette subfamily C member 4
• NCBI Official Symbol: ABCC4
• NCBI Official Synonym Symbols: MRP4; MOATB; MOAT-B
• NCBI Protein Information: multidrug resistance-associated protein 4
• UniProt Protein Name: Multidrug resistance-associated protein 4
• Protein Family: Multidrug resistance-associated protein
• UniProt Gene Name: ABCC4
• UniProt Synonym Gene Names: MRP4; MOAT-B
• UniProt Entry Name: MRP4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This family member plays a role in cellular detoxification as a pump for its substrate, organic anions. It may also function in prostaglandin-mediated cAMP signaling in ciliogenesis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Uniprot Description

ABCC4: a widely-expressed multi-pass membrane protein. An organic anion pump involved in cellular detoxification. Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ABC family

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: basolateral plasma membrane; integral to membrane; membrane; plasma membrane; platelet dense granule membrane

Molecular Function: 15-hydroxyprostaglandin dehydrogenase (NAD+) activity; ATP binding; ATPase activity, coupled to transmembrane movement of substances

Biological Process: blood coagulation; cilium biogenesis; platelet activation; platelet degranulation; prostaglandin secretion; transmembrane transport