Typical Testing Data/Standard Curve (for reference only)
Human Cortisol ELISA Kit
Cortisol (Saliva) Human ELISA Kit
Reactivity
Human
Synonyms
Cortisol; Cortisol (Saliva) Human ELISA Kit; Cortisol elisa kit
Reactivity
Human
Application Notes
Quantitative detection of Cortisol
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Cortisol elisa kit
Background/Introduction: Cortisol (Saliva) Enzyme-Linked Immunosorbent Assay (ELISA) Kit is an in vitro assay for the quantitative measurement of Cortisol in saliva. Cortisol is a steroid hormone released from the adrenal cortex in response to the hormone ACTH. It increases blood pressure and blood sugar levels, and suppresses the immune system. Cortisol acts through specific intracellular receptors and has effects on numerous physiologic systems, including immune system, glucose-counter regulation, vascular tone, substrate utilization, and bone metabolism. It exists in the blood as either a free form or bound to corticosteroid-binding globulin (CBG). While only 1-10% of Cortisol in the blood exists in the unbound/free form, this fraction represents the active pool of Cortisol eliciting the biological response, and is capable of diffusing into target tissues and saliva. Studies consistently report high correlation between salivary and serum cortisol levels, indicating that salivary cortisol can reliably estimate serum Cortisol levels. Cortisol (Saliva) ELISA kit is a competitive enzyme immunoassay kit. The assay employs an antibody specific for cortisol coated on a 96-well plate. Cortisol in a sample and HRP labeled Cortisol compete for binding to the immobilized antibody. After simultaneous incubation, the excess reagents are washed away and a TMB substrate is added to the wells. Color develops in proportion to the amount of bound Cortisol HRP conjugate, and the intensity is inversely proportional to the concentration of Cortisol in the sample. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Sensitivity of the kit is 1 ng/ml and detection range is from 1 ng/ml to 100 ng/ml. The cross-reactivity of this ELISA assay is 6.8% for prednisolone, 4.22% for Cortisone, and <0.1% for other hormones, including 11-deoxycortisol, estradiol, testosterone, and progesterone.
Product Categories/Family for Cortisol elisa kit
Similar Products
Product Notes
The Human Cortisol (Catalog #AAA843397) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA843397 ELISA Kit recognizes Human Cortisol. Quantitative detection of Cortisol. Researchers should empirically determine the suitability of the Cortisol for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cortisol, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
