Rabbit DEDD2 Polyclonal Antibody | anti-DEDD2 antibody

DEDD2 Antibody

Cat. Num. AAA150411

• Gene Names: DEDD2; FLAME-3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: DEDD2 Antibody is immunoaffinity chromatography purified IgG.
• Synonyms: DEDD2; Polyclonal Antibody; DEDD2 Antibody; FLAME-3; FLAME3; PSEC0004; DED-containing protein FLAME-3; death effector domain containing 2; anti-DEDD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: DEDD2 Antibody is immunoaffinity chromatography purified IgG.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 326

• Applicable Applications for anti-DEDD2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: DEDD2 antibody can be used for detection of DEDD2 by Western blot at 0.5 to 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: DEDD2 antibody was raised against a peptide corresponding to 11 amino acids near the amino-terminus of human DEDD2.
• Buffer: DEDD2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: DEDD2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of DEDD2 in RAW264.7 cell lysate with DEDD2 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.)

Related Product Information for anti-DEDD2 antibody

DEDD2 Antibody: Apoptotic signals are often triggered by cell surface death receptors through protein-protein interactions mediated by conserved domains such as the death effector domain. A novel death effector domain (DED)-containing protein, DEDD2, has been recently identified and its over-expression in transfected cells induces moderate apoptosis and results in substantial sensitization to apoptosis induced by Fas, TRAIL, and FADD. More recently, work has shown that DEDD2 can bind caspase-8 and -10 in addition to FLIP but not FADD. Like the related protein DEDD, DEDD2 translocates from the cytosol to the nucleus upon induction of apoptosis, and it has been suggested that DEDD2 may target caspase-8 to the nucleus and that DEDD2 thus plays a critical role in death receptor-induced apoptosis. At least two alternatively spliced transcript variants encoding distinct isoforms have been found for DEDD2.

NCBI and Uniprot Product Information

• NCBI GI #: 19923050
• NCBI GeneID: 162989
• NCBI Accession #: NP_579874
• NCBI GenBank Nucleotide #: NM_133328.3
• UniProt Accession #: Q8WXF8; Q8NBR2; Q8NES1; Q8TAA8; Q96D35
• Molecular Weight: 35,577 Da
• NCBI Official Full Name: DNA-binding death effector domain-containing protein 2 isoform 1
• NCBI Official Synonym Full Names: death effector domain containing 2
• NCBI Official Symbol: DEDD2
• NCBI Official Synonym Symbols: FLAME-3
• NCBI Protein Information: DNA-binding death effector domain-containing protein 2; DED-containing protein FLAME-3; FADD-like anti-apoptotic molecule 3
• UniProt Protein Name: DNA-binding death effector domain-containing protein 2
• Protein Family: DNA-binding death effector domain-containing protein
• UniProt Gene Name: DEDD2
• UniProt Synonym Gene Names: FLAME3
• UniProt Entry Name: DEDD2_HUMAN

NCBI Description

This gene encodes a nuclear-localized protein containing a death effector domain (DED). The encoded protein may regulate the trafficking of caspases and other proteins into the nucleus during death receptor-induced apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]

Uniprot Description

DEDD2: May play a critical role in death receptor-induced apoptosis and may target CASP8 and CASP10 to the nucleus. May regulate degradation of intermediate filaments during apoptosis. May play a role in the general transcription machinery in the nucleus and might be an important regulator of the activity of GTF3C3. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: nucleoplasm; nucleolus

Molecular Function: protein binding; receptor signaling complex scaffold activity; DNA binding

Biological Process: RNA processing; induction of apoptosis via death domain receptors; transcription, DNA-dependent; apoptotic nuclear changes; rRNA catabolic process; cellular homeostasis; negative regulation of transcription, DNA-dependent