Rabbit C4B Polyclonal Antibody | anti-C4A antibody

C4B Antibody (Center)

Cat. Num. AAA9208858

• Gene Names: C4B; CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3
• Reactivity: Tested: Human; Predicted: Human
• Applications: Western Blot, ELISA
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: C4B; Polyclonal Antibody; C4B Antibody (Center); Complement C4-A; Acidic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2; Complement C4 beta chain; Complement C4-A alpha chain; C4a anaphylatoxin; C4b-A; C4d-A; Complement C4 gamma chain; C4A; CO4; CPAMD2; anti-C4A antibody

• Host: Rabbit
• Reactivity: Tested: Human; Predicted: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This C4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 655-684 amino acids from the Central region of human C4B.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 655-684
• Sequence Length: 1,698

• Applicable Applications for anti-C4A antibody: Western Blot (WB), ELISA
• Application Notes: WB~~1:1000
• Function: Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.
• Cellular Location: Secreted. Cell junction, synapse. Cell projection, axon. Cell projection, dendrite
• Tissue Location: Complement component C4 is expressed at highest levels in the liver, at moderate levels in the adrenal cortex, adrenal medulla, thyroid gland,and the kidney, and at lowest levels in the heart, ovary, small intestine, thymus, pancreas and spleen. The extra-hepatic sites of expression may be important for the local protection and inflammatory response
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-C4 Antibody (Center) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 193 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-C4A antibody

This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9.

Product Categories/Family for anti-C4A antibody

Immunology

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Fernando, M.M., et al. Hum. Mutat. 31(7):866-874(2010)
Mostafa, G.A., et al. J. Neuroimmunol. 223 (1-2), 115-119 (2010) :
Hamad, O.A., et al. J. Immunol. 184(5):2686-2692(2010)
Mougey, R. Immunohematology 26(1):30-38(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 178557739
• NCBI GeneID: 721
• NCBI Accession #: NP_001002029.3; NP_001239133.1; NP_009224.2
• NCBI GenBank Nucleotide #: NM_001002029.3
• UniProt Accession #: P0C0L4; P01028; P78445; Q13160; Q13906; A6H8M8; A6NHJ5; A7E2V2; B0QZR6; B0V2C8; B2RUT6; B7ZVZ6; P0C0L5
• Molecular Weight: 192785
• NCBI Official Full Name: complement C4-B preproprotein
• NCBI Official Synonym Full Names: complement component 4B (Chido blood group)
• NCBI Official Symbol: C4B
• NCBI Official Synonym Symbols: CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3
• NCBI Protein Information: complement C4-B
• UniProt Protein Name: Complement C4-A
• Protein Family: Complement C4
• UniProt Gene Name: C4A
• UniProt Synonym Gene Names: CO4; CPAMD2
• UniProt Entry Name: CO4A_HUMAN

NCBI Description

This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9. [provided by RefSeq, Jul 2008]

Uniprot Description

C4A: C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway. Defects in C4A are the cause of complement component 4A deficiency (C4AD). A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis. Defects in C4A are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Interindividual copy- number variation (CNV) of complement component C4 and associated polymorphisms result in different susceptibilities to SLE. The risk of SLE susceptibility has been shown to be significantly increased among subjects with only two copies of total C4. A high copy number is a protective factor against SLE.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular region; plasma membrane

Molecular Function: complement component C1q binding; endopeptidase inhibitor activity

Biological Process: regulation of complement activation; innate immune response; inflammatory response; complement activation, classical pathway; complement activation

Disease: Complement Component 4a Deficiency; Blood Group, Chido/rodgers System