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Western Blot (WB) ((2ug/ml) staining of Rat Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat Cyp2d5/Cyp2d10 Polyclonal Antibody | anti-Cyp2d5 antibody

Goat anti-Cyp2d5/Cyp2d10 (rat) Antibody

Gene Names
Cyp2d5; Cyp2d10
Reactivity
Tested: Rat; Expected from sequence similarity: Rat
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Cyp2d5/Cyp2d10; Polyclonal Antibody; Goat anti-Cyp2d5/Cyp2d10 (rat) Antibody; Cyp2d5; cytochrome P450; family 2; subfamily d; polypeptide 5; Cyp2d10; MGC108540; CYPIID10; P450-CMF1B; P450-DB5; cytochrome P450 2D10; polypeptide 10; cytochrome P450-CMF1B; cytochrome P450-DB5; cytochrome P450CMF; Cyp2d5 antibody; polypeptide 5 antibody; Cyp2d10 antibody; MGC108540 antibody; CYPIID10 antibody; P450-CMF1B antibody; P450-DB5 antibody; cytochrome P450 2D10 antibody; polypeptide 10 antibody; cytochrome P450-DB5 antibody; cytochrome P450CMF antibody; Cyp2d5 / Cyp2d10 (rat); anti-Cyp2d5 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Rat; Expected from sequence similarity: Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
CDAFTAQNGRSINPK
Sequence Length
504
Applicable Applications for anti-Cyp2d5 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 65kDa band observed in Rat Spleen lysates (calculated MW of 57.1kDa according to NP_775426.1). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence CDAFTAQNGRSINPK, from the internal region of the protein sequence according to NP_775426.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((2ug/ml) staining of Rat Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((2ug/ml) staining of Rat Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,076 Da
NCBI Official Full Name
cytochrome P450 2D10
NCBI Official Synonym Full Names
cytochrome P450, family 2, subfamily d, polypeptide 5
NCBI Official Symbol
Cyp2d5
NCBI Official Synonym Symbols
Cyp2d10
NCBI Protein Information
cytochrome P450 2D10
UniProt Protein Name
Cytochrome P450 2D10
UniProt Gene Name
Cyp2d10
UniProt Synonym Gene Names
Cyp2d-10; Cyp2d5
UniProt Entry Name
CP2DA_RAT

NCBI Description

member of a P450 IID gene subfamily that may have been maintained through gene conversion [RGD, Feb 2006]

Uniprot Description

CYP2D5: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.14.1; Oxidoreductase

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: arachidonic acid epoxygenase activity; heme binding; iron ion binding; monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; steroid hydroxylase activity

Biological Process: arachidonic acid metabolic process

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Product Notes

The Cyp2d5 cyp2d10 (Catalog #AAA422669) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Cyp2d5/Cyp2d10 (rat) Antibody reacts with Tested: Rat; Expected from sequence similarity: Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cyp2d5/Cyp2d10 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 65kDa band observed in Rat Spleen lysates (calculated MW of 57.1kDa according to NP_775426.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the Cyp2d5 cyp2d10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CDAFTAQNGR SINPK. It is sometimes possible for the material contained within the vial of "Cyp2d5/Cyp2d10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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