Goat OCT6 Polyclonal Antibody | anti-POU3F1 antibody

Goat anti-OCT6 Antibody

Cat. Num. AAA422765

• Gene Names: POU3F1; OCT6; OTF6; SCIP
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: OCT6; Polyclonal Antibody; Goat anti-OCT6 Antibody; Goat Anti-OCT6 / POU3F1 Antibody; POU3F1; POU class 3 homeobox 1; OTF6; SCIP; POU domain; class 3; transcription factor 1; octamer-binding transcription factor 6; POU3F1 antibody; POU class 3 homeobox 1 antibody; OCT6 antibody; OTF6 antibody; SCIP antibody; transcription factor 1 antibody; octamer-binding transcription factor 6 antibody; anti-POU3F1 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: HHALHEDGHEAQLE
• Sequence Length: 451

• Applicable Applications for anti-POU3F1 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: Approx 50kDa band observed in Human Ovary, Placenta and Umbilical Cord lysates (calculated MW of 45.5kDa according to NP_002690.3). Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence C-HHALHEDGHEAQLE, from the internal region of the protein sequence according to NP_002690.3.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((2ug/ml) staining of Human Umbilical Cord lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 110624765
• NCBI GeneID: 5453
• NCBI Accession #: NP_002690.3
• NCBI GenBank Nucleotide #: NM_002699.3
• Molecular Weight: 45,496 Da
• NCBI Official Full Name: POU domain, class 3, transcription factor 1
• NCBI Official Synonym Full Names: POU class 3 homeobox 1
• NCBI Official Symbol: POU3F1
• NCBI Official Synonym Symbols: OCT6; OTF6; SCIP
• NCBI Protein Information: POU domain, class 3, transcription factor 1
• UniProt Protein Name: POU domain, class 3, transcription factor 1
• Protein Family: POU domain, class 3, transcription factor
• UniProt Gene Name: POU3F1
• UniProt Synonym Gene Names: OCT6; OTF6; Oct-6; OTF-6
• UniProt Entry Name: PO3F1_HUMAN

Uniprot Description

Oct6: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis. Belongs to the POU transcription factor family. Class- 3 subfamily.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: nucleus; transcription factor complex

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: axon ensheathment; forebrain development; keratinocyte differentiation; myelination in the peripheral nervous system; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; transcription, DNA-dependent