Western Blot (WB)
((1ug/ml) staining of fetal Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Cytochrome P450 1A2 Polyclonal Antibody | anti-Cyp1a2 antibody
Goat anti-Cytochrome P450 1A2 (mouse) Antibody
Western Blot: Approx 60kDa band observed in Mouse fetal Liver lysates (calculated MW of 58.2kDa according to NP_034123.1). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of fetal Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of fetal Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
Uniprot Description
CYP1A2: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O- deethylation of phenacetin. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secondary Metabolites Metabolism - caffeine; Amino Acid Metabolism - tryptophan; Xenobiotic Metabolism - metabolism by cytochrome P450; Cofactor and Vitamin Metabolism - retinol; Lipid Metabolism - linoleic acid; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.14.14.1
Cellular Component: endoplasmic reticulum; intracellular membrane-bound organelle; membrane
Molecular Function: demethylase activity; enzyme binding; heme binding; iron ion binding; metal ion binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
Biological Process: alkaloid metabolic process; aromatic compound metabolic process; cellular respiration; dibenzo-p-dioxin metabolic process; drug catabolic process; drug metabolic process; exogenous drug catabolic process; heterocycle metabolic process; hydrogen peroxide biosynthetic process; lipid metabolic process; lung development; monocarboxylic acid metabolic process; monoterpenoid metabolic process; porphyrin metabolic process; post-embryonic development; regulation of gene expression; steroid catabolic process; steroid metabolic process; toxin biosynthetic process; toxin metabolic process
Research Articles on Cyp1a2
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Product Notes
The Cyp1a2 cyp1a2 (Catalog #AAA422748) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Cytochrome P450 1A2 (mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 1A2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 60kDa band observed in Mouse fetal Liver lysates (calculated MW of 58.2kDa according to NP_034123.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the Cyp1a2 cyp1a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HSENYKDNGG LIPEEK. It is sometimes possible for the material contained within the vial of "Cytochrome P450 1A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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