Western Blot (WB)
((1ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat CHRNA5 Polyclonal Antibody | anti-CHRNA5 antibody
Goat anti-CHRNA5 Antibody
Western Blot: Approx 55kDa band observed in lysates of cell line KELLY (calculated MW of 53.1kDa according to NP_000736.2). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in this gene have been linked to susceptibility to lung cancer type 2 (LNCR2).[provided by RefSeq, Jun 2010]
Uniprot Description
nAChRA5: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 5/CHRNA5 sub-subfamily.
Protein type: Membrane protein, integral; Channel, ligand-gated; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 15q24
Cellular Component: cell junction; cell soma; dendrite; nicotinic acetylcholine-gated receptor-channel complex; plasma membrane; postsynaptic membrane
Molecular Function: acetylcholine binding; acetylcholine receptor activity; ligand-gated ion channel activity; nicotinic acetylcholine-activated cation-selective channel activity; protein binding; protein heterodimerization activity
Biological Process: behavioral response to nicotine; neuromuscular synaptic transmission; signal transduction; synaptic transmission; synaptic transmission, cholinergic; transport
Disease: Smoking As A Quantitative Trait Locus 3
Research Articles on CHRNA5
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Product Notes
The CHRNA5 chrna5 (Catalog #AAA422884) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CHRNA5 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHRNA5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 55kDa band observed in lysates of cell line KELLY (calculated MW of 53.1kDa according to NP_000736.2). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the CHRNA5 chrna5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HVDRYFTQKE ET. It is sometimes possible for the material contained within the vial of "CHRNA5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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