Rabbit anti-Human ERAS Polyclonal Antibody | anti-ERAS antibody

ERAS Antibody (N-term)

Cat. Num. AAA9213325

• Gene Names: ERAS; HRAS2; HRASP
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: ERAS; Polyclonal Antibody; ERAS Antibody (N-term); GTPase ERas; E-Ras; Embryonic stem cell-expressed Ras; HRAS2; HRASP; anti-ERAS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human ERAS.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 13-42
• Sequence Length: 233

• Applicable Applications for anti-ERAS antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of ERAS Antibody (N-term) in K562 cell line lysates (35ug/lane). ERAS (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS antibody (N-term) (A28) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Immunofluorescence analysis of anti-ERAS Antibody (N-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of hela cells using ERAS Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ERAS antibody

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells.

Product Categories/Family for anti-ERAS antibody

Cancer; Developmental biology; Signal transduction

References

Kameda,T., Stem Cells 23 (10), 1535-1540 (2005)
Takahashi,K., Nature 423 (6939), 541-545 (2003)
Miyoshi,J., Nucleic Acids Res. 12 (4), 1821-1828 (1984)

NCBI and Uniprot Product Information

• NCBI GI #: 31559833
• NCBI GeneID: 3266
• NCBI Accession #: NP_853510.1
• NCBI GenBank Nucleotide #: NM_181532.3
• UniProt Accession #: Q7Z444
• Molecular Weight: 25287
• NCBI Official Full Name: GTPase ERas
• NCBI Official Synonym Full Names: ES cell expressed Ras
• NCBI Official Symbol: ERAS
• NCBI Official Synonym Symbols: HRAS2; HRASP
• NCBI Protein Information: GTPase ERas
• UniProt Protein Name: GTPase ERas
• Protein Family: GTPase
• UniProt Gene Name: ERAS
• UniProt Synonym Gene Names: HRAS2; HRASP; E-Ras
• UniProt Entry Name: RASE_HUMAN

NCBI Description

This gene encodes a constitutively active member of the small GTPase Ras protein family. The encoded protein activates the phosphatidylinositol 3-kinase signal transduction pathway in undifferentiated stem cells, but is not expressed in differentiated cells. This gene may be involved in cancer and chemotherapy resistance. [provided by RefSeq, Dec 2012]

Uniprot Description

ERas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells. Belongs to the small GTPase superfamily. Ras family.

Protein type: G protein; G protein, monomeric; G protein, monomeric, Ras

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: plasma membrane

Molecular Function: GTP binding

Biological Process: small GTPase mediated signal transduction