ICOS / CD278 Polyclonal Antibody | anti-ICOS / CD278 antibody

Anti-ICOS / CD278 Antibody IHC-plus

Cat. Num. AAA249875

• Gene Names: ICOS; AILIM; CD278; CVID1
• Reactivity: Mouse, Rat, Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Affinity purified
• Synonyms: ICOS / CD278; Polyclonal Antibody; Anti-ICOS / CD278 Antibody IHC-plus; Rabbit Polyclonal (IgG) to Human ICOS / CD278; Human ICOS / CD278; ICOS; AILIM; CVID1; Inducible costimulator; Inducible T-cell co-stimulator; CD278 antigen; Inducible T-cell costimulator; anti-ICOS / CD278 antibody

• Host: ICOS/CD278 antibody was produced in Rabbit.
• Reactivity: Mouse, Rat, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human ICOS
• Purity/Purification: Affinity purified
• Form/Format: PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 199

• Applicable Applications for anti-ICOS / CD278 antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Immunohistochemistry (IHC) 1:50-200; Immunohistochemistry-Paraffin (IHC-P) 1:200 ; Immunofluorescence (IF) 1:50-1:200; Western Blot (WB) 1:500-1:2000; ; Optimal dilution to be determined by the researcher.
• Immunogen Species: ICOS / CD278 antibody was raised against Human
• Immunogen: ICOS/CD278 antibody was raised against recombinant protein of human ICOS.
• Antigen Type: Recombinant protein
• Target Species: ICOS/CD278
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Preparation and Storage: Store at -20°C. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cell using ICOS antibody. Blue: DAPI for nuclear staining.)

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ICOS antibody.)

Related Product Information for anti-ICOS / CD278 antibody

ICOS Antibody, AILIM Antibody, CD278 Antibody, CVID1 Antibody, Inducible costimulator Antibody, Inducible T-cell co-stimulator Antibody, CD278 antigen Antibody, Inducible T-cell costimulator Antibody Description: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10.

NCBI and Uniprot Product Information

• NCBI GI #: 50400701
• NCBI GeneID: 29851
• UniProt Accession #: Q9Y6W8; Q8N6W8
• NCBI Official Full Name: Inducible T-cell costimulator
• NCBI Official Synonym Full Names: inducible T-cell co-stimulator
• NCBI Official Symbol: ICOS
• NCBI Official Synonym Symbols: AILIM; CD278; CVID1
• NCBI Protein Information: inducible T-cell costimulator
• UniProt Protein Name: Inducible T-cell costimulator
• UniProt Gene Name: ICOS
• UniProt Synonym Gene Names: AILIM
• UniProt Entry Name: ICOS_HUMAN

NCBI Description

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

ICOS: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up- regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up- regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre- activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. Defects in ICOS are the cause of immunodeficiency common variable type 1 (CVID1). CVID1 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be lo. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: external side of plasma membrane; extracellular region; integral to plasma membrane; plasma membrane

Biological Process: cell-cell adhesion; immune response; T cell costimulation; T cell tolerance induction

Disease: Immunodeficiency, Common Variable, 1; Immunodeficiency, Common Variable, 2