Mouse anti-Human GALT Monoclonal Antibody | anti-GALT antibody

Mouse Monoclonal [clone 4C11] (IgG2a,k) to Human GALT

Cat. Num. AAA246647

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified from ascites by Protein A.
• Synonyms: GALT; Monoclonal Antibody; Mouse Monoclonal [clone 4C11] (IgG2a; k) to Human GALT; Anti-GALT Antibody (clone 4C11) IHC-plus; Gal-1-P uridylyltransferase; Human GALT; anti-GALT antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 4C11
• Specificity: Human GALT
• Purity/Purification: Purified from ascites by Protein A.
• Form/Format: PBS, pH 7.4
• Concentration: 0.5 mg/mL (varies by lot)
• Sequence Length: 379

• Applicable Applications for anti-GALT antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC; IHC-Paraffin (10 ug/mL)Western Blot; ELISA ; ; Optimal dilution to be determined by the researcher)
• Immunogen Description: GALT (AAH15045, 1 a.a. ~ 380 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: GALT antibody was raised against gALT (AAH15045, 1 a.a. ~ 380 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Antigen Type: Recombinant protein.
• Immunogen Species: GALT antibody was raised against human.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Preparation and Storage: Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-GALT antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(GALT monoclonal antibody clone 4C11 Western blot of GALT expression in K-562.)

ELISA (EIA)

(Detection limit for recombinant GST tagged GALT is approximately 0.3 ng/ml as a capture antibody.)

Related Product Information for anti-GALT antibody

Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined.

NCBI and Uniprot Product Information

• NCBI GI #: 22165416
• NCBI GeneID: 2592
• NCBI Accession #: NP_000146.2
• NCBI GenBank Nucleotide #: NM_000155.3
• UniProt Accession #: P07902; Q14355; Q14356; Q14357; Q14358; Q14359; Q14360; Q14361; Q14363; Q14364; B4E097; E7ET32
• NCBI Official Full Name: galactose-1-phosphate uridylyltransferase isoform 1
• NCBI Official Synonym Full Names: galactose-1-phosphate uridylyltransferase
• NCBI Official Symbol: GALT
• NCBI Protein Information: galactose-1-phosphate uridylyltransferase; gal-1-P uridylyltransferase; galactose-1-phosphate uridyl transferase; UDP-glucose--hexose-1-phosphate uridylyltransferase
• UniProt Protein Name: Galactose-1-phosphate uridylyltransferase
• Protein Family: Gallate transporter
• UniProt Gene Name: GALT
• UniProt Synonym Gene Names: Gal-1-P uridylyltransferase
• UniProt Entry Name: GALT_HUMAN

NCBI Description

Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

GALT: Defects in GALT are the cause of galactosemia (GALCT). Galactosemia is an inherited disorder of galactose metabolism that causes jaundice, cataracts, and mental retardation. Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.

Protein type: Motility/polarity/chemotaxis; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Transferase; Carbohydrate Metabolism - galactose; EC 2.7.7.12

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: Golgi apparatus; cytosol

Molecular Function: zinc ion binding; UDP-glucose:hexose-1-phosphate uridylyltransferase activity

Biological Process: galactose catabolic process; UDP-glucose catabolic process; carbohydrate metabolic process; galactose metabolic process; pathogenesis

Disease: Galactosemia