Mouse ALPL / Alkaline Phosphatase Monoclonal Antibody | anti-ALPL antibody

Mouse Monoclonal (IgG1) to Human ALPL / Alkaline Phosphatase

Cat. Num. AAA245455

• Gene Names: ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
• Reactivity: Bovine, Human
• Purity: Protein A Purified
• Synonyms: ALPL / Alkaline Phosphatase; Monoclonal Antibody; Mouse Monoclonal (IgG1) to Human ALPL / Alkaline Phosphatase; Anti-ALPL / Alkaline Phosphatase Antibody IHC-plus; ALPL; Alkaline phosphomonoesterase; AP-TNAP; Alkaline phosphatase liver; APTNAP; Glycerophosphatase; Tissue-nonspecific ALP; TNAP; HOPS; Liver alkaline phosphatase; TNSALP; Human ALPL; Alkaline Phosphatase; anti-ALPL antibody

• Host: Mouse
• Reactivity: Bovine, Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Recognizes Alkaline Phosphatase from human bone. Species cross-reactivity: Alkaline phosphatase from human placenta and calf intestine.
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2, 0.09% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 524

• Application Notes: Immunohistochemistry : MBS245455 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed-parafiin embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antiodoy, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm specifity. The optimal working concentration for MBS245455 was determined to be 10 ug/ml. Suitable for use in ELISA and Western Blot: Stains a band of bone AP at ~90kD.
• Target Species: ALP/Alkaline Phosphatase
• Immunogen Species: APL/ Alkaline Phosphatase antibody was raised against Human.
• Immunogen: ALPL/Alkaline Phosphatease antibody was raised against purified alkaline phosphatase(native)from human bone.
• Antigen Type: Purified protein
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-Bone Alkaline Phosphatase antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-Bone Alkaline Phosphatase antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-ALPL antibody

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme.

NCBI and Uniprot Product Information

• NCBI GI #: 116734717
• NCBI GeneID: 249
• NCBI Accession #: NP_000469.3
• NCBI GenBank Nucleotide #: NM_000478.4
• UniProt Accession #: P05186; O75090; Q2TAI7; Q59EJ7; Q5BKZ5; Q5VTG5; Q6NZI8; Q8WU32; A1A4E7; B2RMP8; B7Z387; B7Z4Y6
• NCBI Official Full Name: alkaline phosphatase, tissue-nonspecific isozyme isoform 1
• NCBI Official Synonym Full Names: alkaline phosphatase, liver/bone/kidney
• NCBI Official Symbol: ALPL
• NCBI Official Synonym Symbols: HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
• NCBI Protein Information: alkaline phosphatase, tissue-nonspecific isozyme; glycerophosphatase; tissue-nonspecific ALP; alkaline phosphomonoesterase; liver/bone/kidney-type alkaline phosphatase; alkaline phosphatase liver/bone/kidney isozyme
• UniProt Protein Name: Alkaline phosphatase, tissue-nonspecific isozyme
• Protein Family: Alkaline phosphatase
• UniProt Gene Name: ALPL
• UniProt Synonym Gene Names: AP-TNAP; TNSALP
• UniProt Entry Name: PPBT_HUMAN

NCBI Description

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]

Uniprot Description

ALPL: This isozyme may play a role in skeletal mineralization. Defects in ALPL are a cause of hypophosphatasia (HOPS). HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto). Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC). Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI). Belongs to the alkaline phosphatase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cofactor and Vitamin Metabolism - folate biosynthesis; Phosphatase (non-protein); Motility/polarity/chemotaxis; Membrane protein, GPI anchor; EC 3.1.3.1

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: extracellular matrix; extracellular space; membrane; integral to membrane; plasma membrane

Molecular Function: protein binding; pyrophosphatase activity; alkaline phosphatase activity; metal ion binding

Biological Process: osteoblast differentiation; response to antibiotic; response to vitamin D; dephosphorylation; response to glucocorticoid stimulus; reproductive developmental process; response to lipopolysaccharide; skeletal development; endochondral ossification

Disease: Hypophosphatasia, Infantile; Hypophosphatasia, Adult; Hypophosphatasia, Childhood