Rabbit anti-Human CDH1 / E Cadherin Polyclonal Antibody | anti-CDH1 antibody

Rabbit Polyclonal (IgG) to Human CDH1 / E Cadherin

Cat. Num. AAA244515

• Gene Names: CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CDH1 / E Cadherin; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human CDH1 / E Cadherin; Anti-CDH1 / E Cadherin Antibody (aa1-50) IHC-plus; CDH1; CD324; CDHE; Cell-CAM 120/80; E Cadherin; E-Cadherin; ECAD; Epithelial cadherin; Arc-1; UVO; Cadherin-1; CAM 120/80; CD324 antigen; LCAM; Uvomorulin; Human CDH1; anti-CDH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: E-cadherin Antibody detects endogenous levels of total E-cadherin protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 882

• Applicable Applications for anti-CDH1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:40000), IF (1:100 - 1:500), IHC-P (5 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human E-cadherin (aa1-50).
• Immunogen: CDH1 / E Cadherin antibody was raised against synthetic peptide from human E-cadherin (aa1-50).
• Antigen Modification: aa1-50
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-E Cadherin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of A549 cells, using E-cadherin Antibody. The picture on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot of extracts from 293 cells, using E-cadherin Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-CDH1 antibody

E Cadherin (CDH1) is a classical cadherin from the cadherin superfamily. It is a calcium dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis.

Product Categories/Family for anti-CDH1 antibody

Family: Cadherin

NCBI and Uniprot Product Information

• NCBI GI #: 4757960
• NCBI GeneID: 999
• NCBI Accession #: NP_004351.1
• NCBI GenBank Nucleotide #: NM_004360.3
• UniProt Accession #: P12830; Q13799; Q14216; Q15855; Q16194; Q4PJ14; Q9UII8; A8K1U7
• Molecular Weight: 90,942 Da
• NCBI Official Full Name: cadherin-1 preproprotein
• NCBI Official Synonym Full Names: cadherin 1, type 1, E-cadherin (epithelial)
• NCBI Official Symbol: CDH1
• NCBI Official Synonym Symbols: UVO; CDHE; ECAD; LCAM; Arc-1; CD324
• NCBI Protein Information: cadherin-1; CAM 120/80; E-Cadherin; uvomorulin; cell-CAM 120/80; epithelial cadherin; cadherin 1, E-cadherin (epithelial); calcium-dependent adhesion protein, epithelial
• UniProt Protein Name: Cadherin-1
• Protein Family: Cadherin
• UniProt Gene Name: CDH1
• UniProt Synonym Gene Names: CDHE; UVO; E-cadherin
• UniProt Entry Name: CADH1_HUMAN

NCBI Description

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq, Jul 2008]

Uniprot Description

CDH1: a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Has a potent invasive suppressor role.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: apical junction complex; internal side of plasma membrane; cell surface; focal adhesion; lateral loop; basolateral plasma membrane; integral to membrane; extracellular region; trans-Golgi network; catenin complex; actin cytoskeleton; cell-cell adherens junction; perinuclear region of cytoplasm; apical part of cell; cytoplasm; plasma membrane; nerve terminal; cell junction; lateral plasma membrane; endosome

Molecular Function: protein domain specific binding; protein binding; gamma-catenin binding; beta-catenin binding; GTPase activating protein binding; ankyrin binding; calcium ion binding; cell adhesion molecule binding; glycoprotein binding; protein phosphatase binding

Biological Process: response to drug; intercellular junction assembly and maintenance; extracellular matrix organization and biogenesis; regulation of immune response; apoptosis; positive regulation of transcription, DNA-dependent; response to toxin; regulation of caspase activity; trophectodermal cell differentiation; regulation of water loss via skin; extracellular matrix disassembly; cell-cell adhesion; sensory perception of sound; synaptogenesis; pituitary gland development; protein metabolic process; calcium-dependent cell-cell adhesion; positive regulation of transcription factor import into nucleus; negative regulation of cell-cell adhesion; homophilic cell adhesion; protein homooligomerization; neurite development; negative regulation of epithelial cell proliferation; cell structure disassembly during apoptosis

Disease: Gastric Cancer, Hereditary Diffuse; Prostate Cancer; Breast Cancer; Ovarian Cancer; Endometrial Cancer