Rabbit anti-Human TNFSF4 / OX40L Polyclonal Antibody | anti-TNFSF4 / OX40L antibody

Anti-TNFSF4 / OX40L Antibody (aa102-131) IHC-plus

Cat. Num. AAA249958

• Gene Names: TNFSF4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
• Reactivity: Human
• Applications: Immunohistochemistry, Immunohistochemistry, Western Blot
• Purity: Protein A purified
• Synonyms: TNFSF4 / OX40L; Polyclonal Antibody; Anti-TNFSF4 / OX40L Antibody (aa102-131) IHC-plus; Rabbit Polyclonal to Human TNFSF4 / OX40L; Human TNFSF4 / OX40L; CD134 ligand; CD134L; CD252 antigen; Glycoprotein Gp34; OX-40L; OX40 ligand; TXGP1; OX4OL; CD252; gp34; OX40 antigen ligand; anti-TNFSF4 / OX40L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: This TNFSF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-131 amino acids from the Central region of human TNFSF4.
• Purity/Purification: Protein A purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 183

• Applicable Applications for anti-TNFSF4 / OX40L antibody: Immunohistochemistry (IHC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P (1:100); WB (1:1000)
• Immunogen Species: TNFS4/OX40L antibody was raised against Human.
• Family/Subfamily: TNF/not assigned-TNF
• Epitope: aa102-131
• Target: TNFSF4/OX40L
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 6 months.; For long term storage store at -20°C.

Immunohistochemistry (IHC)

(Anti-TNFSF4 / OX40L antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-TNFSF4 / OX40L antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Western Blot (WB)

(TNFSF4 Antibody western blot of CEM cell line lysates (35 ug/lane). The TNFSF4 antibody detected the TNFSF4 protein (arrow).)

Related Product Information for anti-TNFSF4 / OX40L antibody

TNFSF4 Antibody, CD134 ligand Antibody, CD134L Antibody, CD252 antigen Antibody, Glycoprotein Gp34 Antibody, OX-40L Antibody, OX40 ligand Antibody, TXGP1 Antibody, OX40L Antibody, OX4OL Antibody, CD252 Antibody, gp34 Antibody, OX40 antigen ligand Antibody Description: TNFSF4 / OX40L is a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 4507603
• NCBI GeneID: 7292
• NCBI Accession #: NP_003317.1
• NCBI GenBank Nucleotide #: NM_003326.4
• UniProt Accession #: P23510; Q5JZA5; Q8IV74; Q9HCN9
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 4 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor superfamily member 4
• NCBI Official Symbol: TNFSF4
• NCBI Official Synonym Symbols: GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 4
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 4
• UniProt Gene Name: TNFSF4
• UniProt Synonym Gene Names: TXGP1; OX40L
• UniProt Entry Name: TNFL4_HUMAN

NCBI Description

This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

TNFSF4: Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production. Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T-cell-APC interaction and the functional consequences of T-cell activation, thereby destabilizing peripheral tolerance. Belongs to the tumor necrosis factor family.

Protein type: Cytokine; Cell cycle regulation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: cell surface; extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: cytokine activity; receptor binding; tumor necrosis factor receptor binding; tumor necrosis factor receptor superfamily binding

Biological Process: acute inflammatory response; cholesterol metabolic process; defense response to nematode; immune response; inflammatory response; negative regulation of cytokine secretion; negative regulation of interferon-gamma production; negative regulation of interleukin-17 production; negative regulation of regulatory T cell differentiation; negative regulation of T-helper 1 cell differentiation; negative regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; positive regulation of activated T cell proliferation; positive regulation of alpha-beta T cell proliferation; positive regulation of B cell activation; positive regulation of CD4-positive, alpha beta T cell differentiation; positive regulation of cell proliferation; positive regulation of immunoglobulin mediated immune response; positive regulation of immunoglobulin secretion; positive regulation of inflammatory response; positive regulation of interferon-gamma production; positive regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of interleukin-13 production; positive regulation of interleukin-2 production; positive regulation of interleukin-4 production; positive regulation of interleukin-6 production; positive regulation of memory T cell differentiation; positive regulation of T cell cytokine production; positive regulation of T cell proliferation; positive regulation of T-helper 2 cell differentiation; positive regulation of T-helper 2 type immune response; regulation of adaptive immune response; regulation of inflammatory response; response to virus; signal transduction; T cell proliferation; tumor necrosis factor-mediated signaling pathway

Disease: Myocardial Infarction, Susceptibility To