Western Blot (WB)
((1ug/ml) staining of Mouse Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat CCP5 Polyclonal Antibody | anti-Agbl5 antibody
Goat anti-CCP5 (mouse) Antibody
Gene Names
Agbl5; CCP5; 4930455N08; 9430057O19Rik
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse
Applications
ELISA, Western Blot, Flow Cytometry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
CCP5; Polyclonal Antibody; Goat anti-CCP5 (mouse) Antibody; Agbl5; ATP/GTP binding protein-like 5; 4930455N08; 9430057O19Rik; ATP/GTP-binding protein-like 5; OTTMUSP00000025344; OTTMUSP00000025346; OTTMUSP00000025347; OTTMUSP00000025348; OTTMUSP00000025350; OTTMUSP00000025351; carboxypeptidase 5; cytosolic; Agbl5 antibody; ATP/GTP binding protein-like 5 antibody; 4930455N08 antibody; 9430057O19Rik antibody; CCP5 antibody; ATP/GTP-binding protein-like 5 antibody; OTTMUSP00000025344 antibody; OTTMUSP00000025346 antibody; OTTMUSP00000025347 antibody; OTTMUSP00000025350 antibody; OTTMUSP00000025351 antibody; cytosolic antibody; cytosolic carboxypeptidase-like protein 5 antibody; OTTMUSP00000025348 antibody; CCP5 (mouse); anti-Agbl5 antibody
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
CFSKPEEAGSHVE
Sequence Length
846
Applicable Applications for anti-Agbl5 antibody
Peptide ELISA (EIA), Western Blot (WB), Flow Cytometry (FC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 100kDa band observed in Mouse Skin lysates (calculated MW of 93.5kDa according to NP_001041657.1). Recommended concentration: 1-3ug/ml.
Western Blot: Approx 100kDa band observed in Mouse Skin lysates (calculated MW of 93.5kDa according to NP_001041657.1). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence CFSKPEEAGSHVE, from the internal region of the protein sequence according to NP_001041657.1.
Epitope
Internal region
Note
This antibody is expected to recognize reported isoform 3 (NP_001041657.1) only.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of Mouse Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Mouse Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Flow Cytometry (FC)
(MBS422662 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Flow Cytometry (FC)
(MBS422662 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
cytosolic carboxypeptidase-like protein 5 isoform 3
NCBI Official Synonym Full Names
ATP/GTP binding protein-like 5
NCBI Official Symbol
Agbl5
NCBI Official Synonym Symbols
CCP5; 4930455N08; 9430057O19Rik
NCBI Protein Information
cytosolic carboxypeptidase-like protein 5
UniProt Protein Name
Cytosolic carboxypeptidase-like protein 5
Protein Family
UniProt Gene Name
Agbl5
UniProt Synonym Gene Names
Ccp5
UniProt Entry Name
CBPC5_MOUSE
Uniprot Description
AGBL5: Metallocarboxypeptidase that mediates tubulin deglutamylation. Specifically catalyzes the deglutamylation of the branching point glutamate side chains generated by post- translational glutamylation in proteins such as tubulins. In contrast, it is not able to act as a long-chain deglutamylase that shortens long polyglutamate chains, a process catalyzed by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6. Belongs to the peptidase M14 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.17.-; Protease
Cellular Component: cytoplasm; cytoskeleton; cytosol; midbody; nucleus
Molecular Function: carboxypeptidase activity; hydrolase activity; metal ion binding; metallocarboxypeptidase activity; metallopeptidase activity; peptidase activity; tubulin binding; zinc ion binding
Biological Process: proteolysis
Research Articles on Agbl5
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Product Notes
The Agbl5 agbl5 (Catalog #AAA422662) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CCP5 (mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CCP5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Flow Cytometry (FC). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 100kDa band observed in Mouse Skin lysates (calculated MW of 93.5kDa according to NP_001041657.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the Agbl5 agbl5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CFSKPEEAGS HVE. It is sometimes possible for the material contained within the vial of "CCP5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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