Western Blot (WB)
((2ug/ml) staining of Human Breast cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat NLRX1/NOD9 Polyclonal Antibody | anti-NLRX1 antibody
Goat anti-NLRX1/NOD9 Antibody
Western Blot: Approx 100kDa band observed in Human Breast cancer lysates (calculated MW of 108kDa according to NP_078894.2 and 102kDa according to NP_733840.1). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((2ug/ml) staining of Human Breast cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of Human Breast cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the NLR family and localizes to the outer mitochondrial membrane. The encoded protein is a regulator of mitochondrial antivirus responses. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2013]
Uniprot Description
NLRX1: Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010). Has no inhibitory function on NF- Kappa-B and type 1 interferon signaling pathways, but enhances NF- Kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313). Interacts with MAVS. Ubiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HeLa, HEK293T, THP-1, HL-60, Raji, and Jurkat cell lines. Belongs to the NLRP family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: mitochondrial outer membrane
Molecular Function: ATP binding; protein binding
Biological Process: innate immune response; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of inflammatory response; negative regulation of innate immune response; negative regulation of interferon type I production; negative regulation of interferon-beta production; negative regulation of interleukin-6 production; viral reproduction
Research Articles on NLRX1
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Product Notes
The NLRX1 nlrx1 (Catalog #AAA422323) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NLRX1/NOD9 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NLRX1/NOD9 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 100kDa band observed in Human Breast cancer lysates (calculated MW of 108kDa according to NP_078894.2 and 102kDa according to NP_733840.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the NLRX1 nlrx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NFSGETLDST DPSN. It is sometimes possible for the material contained within the vial of "NLRX1/NOD9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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