Goat RASSF6 Polyclonal Antibody | anti-RASSF6 antibody

Goat anti-RASSF6 Antibody

Cat. Num. AAA421614

• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: RASSF6; Polyclonal Antibody; Goat anti-RASSF6 Antibody; Ras association (RalGDS/AF-6) domain family 6; DKFZp686K23225; putative RAS binding protein; RASSF6 antibody; Ras association (RalGDS/AF-6) domain family 6 antibody; DKFZp686K23225 antibody; putative RAS binding protein antibody; anti-RASSF6 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: SNTLKPHAKDEPDS
• Sequence Length: 337

• Applicable Applications for anti-RASSF6 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: Approx 45kDa band observed in lysates of cell line HepG2 (calculated MW of 43.4kDa according to NP_958834.1). Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence C-SNTLKPHAKDEPDS, from the internal region of the protein sequence according to NP_803876.1; NP_958834.1.
• Epitope: Internal region
• Note: This antibody is expected to recognise both reported isoforms (NP_803876.1; NP_958834.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 29789443
• NCBI GeneID: 166824
• NCBI Accession #: NP_803876.1; NP_958834.1
• NCBI GenBank Nucleotide #: NM_177532.4
• Molecular Weight: 35,809 Da
• NCBI Official Full Name: ras association domain-containing protein 6 isoform a
• NCBI Official Synonym Full Names: Ras association domain family member 6
• NCBI Official Symbol: RASSF6
• NCBI Protein Information: ras association domain-containing protein 6
• UniProt Protein Name: Ras association domain-containing protein 6
• Protein Family: Ras association domain-containing protein
• UniProt Gene Name: RASSF6
• UniProt Entry Name: RASF6_HUMAN

NCBI Description

This gene encodes a member of the Ras-association domain family (RASSF). Members of this family form the core of a highly conserved tumor suppressor network, the Salvador-Warts-Hippo (SWH) pathway. The protein encoded by this gene is a Ras effector protein that induces apoptosis. A genomic region containing this gene has been linked to susceptibility to viral bronchiolitis. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

RASSF6: Involved in the induction of apoptosis, through both caspase-dependent and caspase-independent pathways. May act as a Ras effector protein. May suppress the serum-induced basal levels of NF-kappa-B. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 4q13.3

Molecular Function: protein binding

Biological Process: apoptosis; signal transduction