Immunoprecipitation (IP)
(CCDC134 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-CCDC134 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CCDC134 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 28 kDa)
Rabbit anti-Human CCDC134 Polyclonal Antibody | anti-CCDC134 antibody
Anti-CCDC134 Antibody, Rabbit Polyclonal
Gene Names
CCDC134; dJ821D11.3
Reactivity
Human
Applications
Western Blot, ELISA, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
CCDC134; Polyclonal Antibody; Anti-CCDC134 Antibody; Rabbit Polyclonal; CCDC134 Antibody; Rabbit PAb; Antigen Affinity Purified; coiled-coil domain containing 134; anti-CCDC134 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CCDC134
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CCDC134 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
ELISA: 1:25,000-1:50,000
IP: 0.2-1uL/mg of lysate
ELISA: 1:25,000-1:50,000
IP: 0.2-1uL/mg of lysate
Immunogen
Recombinant Human CCDC134 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human CCDC134 (rh CCDC134). CCDC134 specific IgG was purified by Human CCDC134 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunoprecipitation (IP)
(CCDC134 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-CCDC134 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CCDC134 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 28 kDa)
Immunoprecipitation (IP)
(CCDC134 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-CCDC134 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CCDC134 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 28 kDa)
Western Blot (WB)
(Anti-CCDC134 rabbit polyclonal antibody at 1:500 dilutionLane A: Hl-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa)
Western Blot (WB)
(Anti-CCDC134 rabbit polyclonal antibody at 1:500 dilutionLane A: Hl-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa)
Related Product Information for anti-CCDC134 antibody
Coiled-coil domain containing 134 (CCDC134) is a 229 amino acids secretory protein. Coiled-coil domain is a motif in which alpha-helix are coiled together. It has been found in many types of proteins, including transcription factors, intermediate filaments and certain tRNA synthetases. Many proteins containing such motif CCDC134 are involved in important biological functions. CCDC134 is also considered as a novel human MAPK-regulating protein that can inhibit the MAPK pathway. This protein significantly inhibite Elk1 transcriptional activity. The coiled-coil domain is a ubiquitous protein motif that is often involved in oligomerization.
References
Huang J, et al. (2007) CCDC134, a novel secretory protein, inhibits activation of ERK and JNK, but not p38 MAPK. Cellular and Molecular Life Sciences. 65(2): 338-49.Kim S, et al. (2011) Genome-wide association study of CSF biomarkers Abeta1-42, t-tau, and p-tau181p in the ADNI cohort. Neurology. 76(1): 69-79.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,561 Da
NCBI Official Full Name
coiled-coil domain-containing protein 134
NCBI Official Synonym Full Names
coiled-coil domain containing 134
NCBI Official Symbol
CCDC134
NCBI Official Synonym Symbols
dJ821D11.3
NCBI Protein Information
coiled-coil domain-containing protein 134
UniProt Protein Name
Coiled-coil domain-containing protein 134
Protein Family
UniProt Gene Name
CCDC134
UniProt Entry Name
CC134_HUMAN
Uniprot Description
CCDC134: Belongs to the UPF0388 family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 22q13.2
Cellular Component: membrane; extracellular region
Research Articles on CCDC134
Similar Products
Product Notes
The CCDC134 ccdc134 (Catalog #AAA8106797) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CCDC134 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCDC134 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the CCDC134 ccdc134 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCDC134, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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