Rabbit anti-Mouse SPARC Monoclonal Antibody | anti-SPARC antibody

Recombinant Anti-SPARC Antibody, Rabbit Monoclonal

Cat. Num. AAA8111659

• Gene Names: Sparc; ON; BM-40
• Reactivity: Mouse
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A
• Synonyms: SPARC; Monoclonal Antibody; Recombinant Anti-SPARC Antibody; Rabbit Monoclonal; Osteonectin/SPARC Antibody; Rabbit MAb; secreted protein; acidic; cysteine-rich (osteonectin); Anti-AA517111 Antibody; Anti-BM-40 Antibody; Anti-ON Antibody; Anti-RP23-465I4.1 Antibody; anti-SPARC antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 001
• Specificity: Mouse Osteonectin/SPARC
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SPARC antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IP: 0.2-1uL/mg of lysate
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Mouse Osteonectin/SPARC protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Osteonectin/SPARC (rM Osteonectin/SPARC).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-SPARC rabbit monoclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:40 kDa)

Immunoprecipitation (IP)

(Mouse SPARC was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-Mouse SPARC rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse SPARC rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 38 kDa)

Related Product Information for anti-SPARC antibody

Secreted protein acidic and rich in cysteine (SPARC), also known as Osteonectin (ON), is a member of the SPARC family. SPARC consists of three domains: an EF-hand domain, a follistatin-like domain and a Kazal-like domain, and each of which has independent activity and unique properties. The activity of SPARC is context-and cell-type-dependent, which is highlighted by the fact that SPARC has shown seemingly contradictory effects on tumor progression in both clinical correlative studies and in animal models. The location of SPARC in the nuclear matrix of certain proliferating cells, but only in the cytosol of postmitotic neurons, indicates potential functions of SPARC as a nuclear protein, which might be involved in the regulation of cell cycle progression and mitosis. It functions not only to modulate cell-cell and cell-matrix interactions, but its de-adhesive and growth inhibitory properties in non-transformed cells have led to studies to assess its role in cancer. Its divergent actions reflect the complexity of this protein, because in certain types of cancers, such as melanomas and gliomas, SPARC is associated with a highly aggressive tumor phenotype, while in others, mainly ovarian, neuroblastomas and colorectal cancers, SPARC may function as a tumor suppressor. Recent studies have also demonstrated a role for SPARC in sensitizing therapy-resistant cancers. Notably, SPARC is linked to human obesity.

References

Yan Q, et al. (1999) SPARC, a matricellular glycoprotein with important biological functions. J Histochem Cytochem. 47(12): 1495-506.Brekken RA, et al. (2000) SPARC, a matricellular protein: at the crossroads of cell-matrix. Matrix Biol. 19(7): 569-80.Tai IT, et al. (2008) SPARC in cancer biology: its role in cancer progression and potential for therapy. Drug Resist Updat. 11(6): 231-46.Podhajcer OL, et al. (2008) The role of the matricellular protein SPARC in the dynamic interaction between the tumor and the host. Cancer Metastasis Rev. 27(3): 523-37.Kos K, et al. (2010) SPARC: a key player in the pathologies associated with obesity and diabetes. Nat Rev Endocrinol. 6(4): 225-35.

NCBI and Uniprot Product Information

• NCBI GI #: 6678077
• NCBI GeneID: 20692
• NCBI Accession #: NP_033268.1
• NCBI GenBank Nucleotide #: NM_009242.5
• UniProt Accession #: P07214
• Molecular Weight: 34,450 Da
• NCBI Official Full Name: SPARC
• NCBI Official Synonym Full Names: secreted acidic cysteine rich glycoprotein
• NCBI Official Symbol: Sparc
• NCBI Official Synonym Symbols: ON; BM-40
• NCBI Protein Information: SPARC
• UniProt Protein Name: SPARC
• Protein Family: SPARC
• UniProt Gene Name: Sparc
• UniProt Synonym Gene Names: BM-40; ON

Uniprot Description

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.