Rabbit anti-Human CAMKI Polyclonal Antibody | anti-CAMK1 antibody

Anti-CAMKI Antibody, Rabbit Polyclonal

Cat. Num. AAA8105987

• Gene Names: CAMK1; CAMKI
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: CAMKI; Polyclonal Antibody; Anti-CAMKI Antibody; Rabbit Polyclonal; CAMKI/CAMK1 Antibody; Rabbit PAb; Antigen Affinity Purified; calcium/calmodulin-dependent protein kinase I; anti-CAMK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CAMKI/CAMK1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CAMK1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IP: 0.2-1uL/mg of lysate
• Immunogen: Recombinant Human CAMKI/CAMK1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CAMKI/CAMK1 (rh CAMKI/CAMK1). CAMKI/CAMK1 specific IgG was purified by Human CAMKI/CAMK1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(CAMK1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate0.5 uL anti-CAMK1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CAMK1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size: 40 kDa)

Western Blot (WB)

(Anti-CAMKII rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:41 kDaObserved band size:45 kDa)

Related Product Information for anti-CAMK1 antibody

Calcium/calmodulin-dependent protein kinase orCaM kinases are serine/threonine-specific protein kinasesthat are primarily regulated by the Calcium/calmodulin complex. These kinases show a memory effect on activation. CaM kinases activity can outlast the intracellular calcium transient that is needed to activate it. Inneurons, this property is important for the induction of synaptic plasticity. Pharmacological inhibition of CaM kinases II blocks the induction oflong-term potentiation. Upon activation, CaM kinases II phosphorylates postsynaptic glutamate receptors and changes the electrical properties of the synapse. Calcium/calmodulin-dependent protein kinase type 1D, also known as CaM kinase I delta, CaM kinase ID, CaMKI-like protein kinase, CKLiK and CAMK1D, is a member of theprotein kinase superfamily and CaMK subfamily. It contains oneprotein kinase domain. CAMK1D is broadly expressed. It is highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. Engineered overexpression of CAMK1D in non-tumorigenic breast epithelial cells led to increased cell proliferation, and molecular and phenotypic alterations indicative of epithelial-mesenchymal transition (EMT), including loss of cell-cell adhesions and increased cell migration and invasion. CAMK1D is a potential therapeutic target with particular relevance to clinically unfavorable basal-like tumors.

Product Categories/Family for anti-CAMK1 antibody

Calcium-binding Proteins and Related Molecules; EGFR Signaling Pathway

References

Lisman, JE. et al., 1985, Proc Natl Acad Sci USA. 82 (9): 3055-7. Bergamaschi, A. et al., 2008, Mol Oncol. 2 (4): 327-39. White RB. et al., 2008, Physiological genomics, 33 (1): 41-9. Schleinitz, D. et al., 2010, Horm Metab Res. 42 (1): 14-22.

NCBI and Uniprot Product Information

• NCBI GI #: 4502553
• NCBI GeneID: 8536
• NCBI Accession #: NP_003647.1
• NCBI GenBank Nucleotide #: NM_003656.4
• UniProt Accession #: Q14012; Q3KPF6
• Molecular Weight: 41,337 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type 1
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase I
• NCBI Official Symbol: CAMK1
• NCBI Official Synonym Symbols: CAMKI
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type 1; caM-KI; caMKI-alpha; caM kinase I alpha
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type 1
• Protein Family: Calcium/calmodulin-dependent serine/threonine-protein kinase
• UniProt Gene Name: CAMK1
• UniProt Synonym Gene Names: CaM-KI; CaMKI-alpha
• UniProt Entry Name: KCC1A_HUMAN

NCBI Description

Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008]

Uniprot Description

CAMK1A: an ubiquitous protein kinase of the CAMK1 family. Activated by Ca(2+)/calmodulin. Must be phosphorylated to be maximally active. Substrates include synapsin I.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.17; CAMK group; CAMK1 family

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: cytoplasm; nucleus

Molecular Function: calmodulin binding; protein binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: positive regulation of syncytium formation by plasma membrane fusion; regulation of muscle cell differentiation; nucleocytoplasmic transport; signal transduction; cell cycle; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein export from nucleus; positive regulation of synapse structural plasticity; regulation of protein localization; regulation of protein binding; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein binding