Western Blot (WB)
(Anti-PMVK rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:21 kDaObserved band size:24 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human PMVK Monoclonal Antibody | anti-PMVK antibody
Recombinant Anti-PMVK Antibody, Rabbit Monoclonal
Gene Names
PMVK; PMK; PMKA; PMKASE; POROK1; HUMPMKI
Reactivity
Human
Applications
Western Blot, ELISA, Immunoprecipitation
Purity
Protein A
Synonyms
PMVK; Monoclonal Antibody; Recombinant Anti-PMVK Antibody; Rabbit Monoclonal; PMVK/phosphomevalonate kinase Antibody; Rabbit MAb; phosphomevalonate kinase; Anti-HUMPMKI Antibody; Anti-PMK Antibody; Anti-PMKA Antibody; Anti-PMKASE Antibody; anti-PMVK antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
014
Specificity
Human PMVK/phosphomevalonate kinase
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PMVK antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
ELISA: 1:25,000-1:50,000
IP: 0.2-1uL/mg of lysate
ELISA: 1:25,000-1:50,000
IP: 0.2-1uL/mg of lysate
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human PMVK/phosphomevalonate kinase Protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human PMVK/phosphomevalonate kinase (rh PMVK/phosphomevalonate kinase).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PMVK rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:21 kDaObserved band size:24 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-PMVK rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:21 kDaObserved band size:24 kDa(We are unsure as to the identity of these extra bands.))
Immunoprecipitation (IP)
(PMVK was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg NIH-3T3 Whole Cell Lysate0.5 uL anti-PMVK rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PMVK rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 21 kDaObserved band size: 21 kDa)
Immunoprecipitation (IP)
(PMVK was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg NIH-3T3 Whole Cell Lysate0.5 uL anti-PMVK rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PMVK rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 21 kDaObserved band size: 21 kDa)
Related Product Information for anti-PMVK antibody
PMVK is a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of PMVK is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis.
References
Chambliss K.L., et al., (1996), Molecular cloning of human phosphomevalonate kinase and identification of a consensus peroxisomal targeting sequence. J. Biol. Chem. 271:17330-17334.Olivier L.M., et al., (1999), Characterization of phosphomevalonate kinase: chromosomal localization, regulation, and subcellular targeting.J. Lipid Res. 40:672-679.Herdendorf T.J., et al., (2006), Phosphomevalonate kinase: functional investigation of the recombinant human enzyme. Biochemistry 45:3235-3242.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,995 Da
NCBI Official Full Name
phosphomevalonate kinase
NCBI Official Synonym Full Names
phosphomevalonate kinase
NCBI Official Symbol
PMVK
NCBI Official Synonym Symbols
PMK; PMKA; PMKASE; POROK1; HUMPMKI
NCBI Protein Information
phosphomevalonate kinase
UniProt Protein Name
Phosphomevalonate kinase
UniProt Gene Name
PMVK
UniProt Synonym Gene Names
PMKI; PMKase; hPMK
UniProt Entry Name
PMVK_HUMAN
Uniprot Description
PMVK: Induced by sterol. Monomer.
Protein type: Kinase, other; EC 2.7.4.2; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis
Chromosomal Location of Human Ortholog: 1q22
Cellular Component: membrane; peroxisome; cytosol
Molecular Function: phosphomevalonate kinase activity; ATP binding
Biological Process: isopentenyl diphosphate biosynthetic process, mevalonate pathway; sterol biosynthetic process; phosphorylation; cholesterol biosynthetic process
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Product Notes
The PMVK pmvk (Catalog #AAA8107440) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-PMVK Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PMVK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the PMVK pmvk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PMVK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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