Testing Data
(Images: G-CSF treatment increases the number of migrated b-endorphin-containing PMN cells in injured nerves. (A-C) Confocal laser scanning microscopy of PMN cells and b-endorphin peptides in the right sciatic nerve of vehicle-treated sham rats (A1-3), vehicle-treated CCI rats (B1-3), and G-CSF treated CCI rats (C1-3) 12 h after CCI, respectively. No apparent PMN cells or b-endorphin peptides were observed in the vehicle-treated sham operation rats. However, at 12 h, the count of b-endorphin-containing PMN cells was significantly higher in the G-CSF-treated CCI rats than in the vehicle-treated CCI rats. Scale bar: 100 mum. (D) Confocal high-power field laser scanning pictures showing a PMN cell with a typical segmented nucleus, which stained positive for b-endorphin peptides. Green: PMN cells; red: b-endorphin (END)-containing cells; blue (DAPI): cell nuclei. Scale bar: 10 mum. (E) The number of b-endorphin-containing PMN cells per section as calculated at different time points. The count of b-endorphin-containing PMN cells was higher in the CCI rats treated with G-CSF (black bars) compared to those treated with vehicle (grey bars) between 12-48 h after nerve injury. Data are shown as the means±SEM, n = 5 per group; one-way ANOVA, *p<0.05, **p<0.01: CCI + G-CSF group compared to CCI + vehicle group; #p<0.05, ##p<0.01: CCI + G-CSF or CCI + vehicle group compared to vehicle-treated sham operation control. doi:10.1371/journal.pone.0043680.g004)
NCBI and Uniprot Product Information
Uniprot Description
Beta-endorphin and Met-enkephalin are endogenous opiates.
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Product Notes
The beta endorphin pomc (Catalog #AAA555202) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The beta endorphin, Antibody, Rabbit reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's beta endorphin can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the beta endorphin pomc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta endorphin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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